Hypertonicity induced apoptosis in HL-60 cells in the presence of intracellular potassium.
Hypertonicity induced apoptosis in HL-60 cells in the presence of intracellular potassium.
复制标题
在细胞内钾存在的情况下，高渗性会诱导 HL-60 细胞凋亡。
DOI：
10.1007/s10495-007-0054-z
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Knauf,PhilipA
中科院分区：
文献类型：
--
作者：
Ghosh,Arko;Keng,PeterC;Knauf,PhilipA
Cell shrinkage is a hallmark of apoptosis. Potassium efflux, which is involved in cell shrinkage, has been previously described as an essential event of apoptosis. This study was designed to address the importance of potassium efflux in hypertonicity (450 mOsm and 600 mOsm) induced apoptosis. We initiated apoptosis in HL-60 cells in hypertonic medium consisting of either high concentrations of NaCl, mannitol or KCl. Apoptotic activity was evaluated based on the DNA content of the cells, annexin-V staining and calcium content. Apoptosis was initiated in hypertonic conditions consisting of high intracellular K+. We demonstrate that apoptosis can occur in the presence of high intracellular potassium contrary to previous predictions.
