Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation
Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation
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DOI：
10.1007/s00411-015-0598-1
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发表时间：
2015-08-01
影响因子：
1.7
通讯作者：
Davidkova, M.
中科院分区：
文献类型：
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作者：
Litvinchuk, Alexandra V.;Vachelova, J.;Davidkova, M.
微核是由染色体碎片或整个染色体在有丝分裂过程中与子核隔绝而形成的小的核外小体。辐射诱发微核的数量反映了染色体损伤的程度，并与入射电离辐射的吸收剂量和质量有关。本研究的目的是确定在30 MeV质子和Co-60γ射线照射下，正常人成纤维细胞中微核的形成作为急性辐射诱发DNA损伤的特异性生物学标志。我们发现，在吸收剂量从1到5Gy时，两种照射方式下，含有微核的双核细胞呈线性增加。但双核细胞微核总数与剂量呈线性二次曲线关系。在人暴露于混合辐射场或高LET辐射的情况下，所有双核细胞中含有微核的双核细胞比例可以作为辐射诱导DNA损伤的良好生物标志物。
Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage.