Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
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DOI：
10.1016/s0021-9258(19)50311-8
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发表时间：
1993-06
期刊：
影响因子：
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通讯作者：
David J. Palmer;J. Helms;C. J. Beckers;C. J. Beckers;L. Orci;J. E. Rothman
中科院分区：
文献类型：
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作者：
David J. Palmer;J. Helms;C. J. Beckers;C. J. Beckers;L. Orci;J. E. Rothman
Coatomer，一个复杂的七种蛋白质，似乎是前体的非网格蛋白涂层高尔基体衍生的囊泡的外壳结构。这种囊泡外壳的另一种成分是胞质蛋白ADP-核糖基化因子（ARF）。ARF与外被体一样，似乎与高尔基体膜可逆地结合。我们现在报告，ARF是所需的coatomer结合高尔基体膜和豆蔻酰化，但不是非豆蔻酰化，ARF是所需的物种。我们利用针对外被体的β亚基（β-COP）的抗体来跟踪外被体结合。ARF和β-COP化学计量地结合到高尔基体膜。ARF依赖性β-COP结合需要膜相关蛋白，是可饱和的，并且在稳定的鸟苷5 '-O-（3-硫代三磷酸）（GTP gamma S）等GTP类似物存在下得到增强。ARF和β-COP依次与高尔基体膜结合，因为β-COP可以与先前用ARF和GTP γ S孵育的再分离的膜结合。我们的结论是，膜结合ARF赋予高尔基体膜的所有要求的特定β-COP结合。
Coatomer, a complex of seven proteins, appears to be the precursor of the coat structure of non-clathrin-coated Golgi-derived vesicles. Another component of this vesicle coat is the cytosolic protein ADP-ribosylation factor (ARF). Like coatomer, ARF appears to reversibly associate with Golgi membranes. We now report that ARF is required for coatomer binding to Golgi membranes and that myristoylated, but not non-myristoylated, ARF is the required species. We utilize an antibody directed against the beta-subunit of coatomer (beta-COP) to follow coatomer binding. ARF and beta-COP bind stoichiometrically to Golgi membranes. ARF-dependent beta-COP binding requires a membrane-associated protein, is saturable, and is enhanced in the presence of stable GTP analogues like guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S). ARF and beta-COP bind sequentially to Golgi membranes, since beta-COP can be bound to reisolated membranes that had been previously incubated with ARF and GTP gamma S. We conclude that membrane-bound ARF confers to Golgi membranes all of the requirements for specific beta-COP binding.