Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products
Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products
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DOI：
10.1159/000338976
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Lademann, J.
中科院分区：
文献类型：
--
作者：
Darvin, M. E.;Koenig, K.;Lademann, J.
氧化锌纳米颗粒 (ZnO NP) 通常用作商业防晒产品中的紫外线过滤剂。文献中对它们对皮肤的渗透进行了深入讨论。在目前的体内研究中，通过多光子断层扫描研究了 ZnO 纳米粒子（大小为 30 nm）对人体皮肤的渗透。基于二次谐波产生和超瑞利散射的非线性效应，分析了ZnO纳米粒子在表皮角质层以及毛囊的沟纹、皱纹和孔口中的分布。该方法可以区分锌的颗粒形式和溶解形式。估计检测限为 0.08 fg/μ m(3)。利用这种敏感性，结果清楚地表明，ZnO NPs 只能渗透到角质层的最外层、皱纹和毛囊的孔口，而不会到达有活力的表皮。版权所有 (C) 2012 S. Karger AG，巴塞尔
Zinc oxide nanoparticles (ZnO NPs) are commonly used as UV filters in commercial sunscreen products. Their penetration into the skin is intensively discussed in the literature. In the present in vivo study, penetration of ZnO NPs (30 nm in size) into human skin was investigated by multiphoton tomography. Based on the non-linear effects of a second harmonic generation and hyper-Rayleigh scattering, the distribution of ZnO NPs in the horny layers of the epidermis, as well as the furrows, wrinkles and orifice of the hair follicles was analyzed. This method permitted distinguishing between the particulate and dissolved forms of Zn. A detection limit of 0.08 fg/mu m(3) was estimated. Taking advantage of this sensitivity, it was clearly shown that ZnO NPs penetrate only into the outermost layers of stratum corneum, furrows and into the orifices of the hair follicles and do not reach the viable epidermis. Copyright (C) 2012 S. Karger AG, Basel