Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil
Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil
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镉污染土壤植物提取过程中景天根际微生物群落结构和代谢功能的距离依赖性变化
DOI：
10.1007/s11356-017-9007-4
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发表时间：
2017
影响因子：
5.8
通讯作者：
Ni Wuzhong
中科院分区：
文献类型：
--
作者：
Yang Wenhao;Zhang Taoxiang;Lin Sen;Ni Wuzhong
微生物群落和活动的恢复对于污染土壤的修复至关重要。植物提取过程中超积累植物根际土壤中微生物群落组成和代谢特征的距离依赖性变化知之甚少。在镉污染的土壤中进行了为期 12 个月的东南景天植物提取实验。预先分层的根箱用于分离亚层根际（距根垫 0-2、2-4、4-6、6-8、8-10 毫米）/散装土壤。通过磷脂脂肪酸（PLFA）和MicroResp™方法分析土壤微生物结构和功能。 0-2mm下层根际土壤中总PLFA生物标志物和特定PLFA生物标志物的浓度以及14种底物（有机碳）的利用率显着增加，但随着距根垫距离的增加而降低。通过不同类 PLFA 的比率（例如真菌/细菌、单不饱和/饱和、革兰氏阳性与革兰氏阴性 (GP/GN) 细菌的比率、环丙基/单烯前体和 19:0 环/18:1ω7c 的比率）测量的微生物结构在 0–2 mm 土壤中发生显着变化。 PLFA含量和底物利用率与pH和Cd的总分数、酸溶性分数和可还原分数呈负相关，而与不稳定碳呈正相关。微生物群落的动态可能是由于根系分泌物和 S 的 Cd 吸收所致。阿尔弗雷迪。这项研究揭示了植物提取对根际微生物群落的刺激和梯度变化，因为微生物群落的反应导致镉浓度、pH 值降低和不稳定碳增加。
The recovery of microbial community and activities is crucial to the remediation of contaminated soils. Distance-dependent variations of microbial community composition and metabolic characteristics in the rhizospheric soil of hyperaccumulator during phytoextraction are poorly understood. A 12-month phytoextraction experiment withSedum alfrediiin a Cd-contaminated soil was conducted. A pre-stratified rhizobox was used for separating sub-layer rhizospheric (0–2, 2–4, 4–6, 6–8, 8–10 mm from the root mat)/bulk soils. Soil microbial structure and function were analyzed by phospholipid fatty acid (PLFA) and MicroResp™ methods. The concentrations of total and specified PLFA biomarkers and the utilization rates for the 14 substrates (organic carbon) in the 0–2-mm sub-layer rhizospheric soil were significantly increased, as well as decreased with the increase in the distance from the root mat. Microbial structure measured by the ratios of different groups of PLFAs such as fungal/bacterial, monounsaturated/saturated, ratios of Gram-positive to Gram-negative (GP/GN) bacterial, and cyclopropyl/monoenoic precursors and 19:0 cyclo/18:1ω7c were significantly changed in the 0–2-mm soil. The PLFA contents and substrate utilization rates were negatively correlated with pH and total, acid-soluble, and reducible fractions of Cd, while positively correlated with labile carbon. The dynamics of microbial community were likely due to root exudates and Cd uptake byS. alfredii. This study revealed the stimulations and gradient changes of rhizosphere microbial community through phytoextraction, as reduced Cd concentration, pH, and increased labile carbons are due to the microbial community responses.