QTL mapping of yield and fiber traits based on a four-way cross population in Gossypium hirsutum L.
QTL mapping of yield and fiber traits based on a four-way cross population in Gossypium hirsutum L.
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DOI：
10.1007/s00122-008-0828-x
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发表时间：
2008-10-01
影响因子：
5.4
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
--
作者：
Qin, Hongde;Guo, Wangzhen;Zhang, Tianzhen
涉及四个不同自交系的四交(4WC)在棉花育种中经常出现。然而，棉花分子标记的连锁分析和数量性状基因座(QTL)定位主要应用于两个自交系的杂交群体，在4WC群体中进行QTL分析的结果很少。本研究以4个不同的陆地棉自交系为材料，构建了棉花产量和纤维品质性状的连锁图谱，并对其进行了QTL定位。构建的连锁图谱有2 85个SSR基因座和1个形态基因座，覆盖棉花基因组总重组长度的42%，覆盖2113.3 cM。共检测到31个QTL，解释的表型方差为5.1~25.8%。24个跨环境通用QTL表现出较高的稳定性，其中6个为环境特异性QTL。确定了几个影响多个性状的基因组片段。讨论了利用4WC进行QTL定位的优势。本研究首次利用陆地棉4WC群体进行QTL定位。本研究结果将加深对陆地棉产量和纤维品质性状遗传基础的理解，并为陆地棉品种群体的进一步分子标记辅助选择奠定基础。
Four-way cross (4WC) involving four different inbred lines frequently appears in the cotton breeding programs. However, linkage analysis and quantitative trait loci (QTL) mapping with molecular markers in cotton has largely been applied to populations derived from a cross between two inbred lines, and few results of QTL dissection were conducted in a 4WC population. In this study, an attempt was made to construct a linkage map and identify QTL for yield and fiber quality traits in 4WC derived from four different inbred lines in Gossypium hirsutum L. A linkage map was constructed with 285 SSR loci and one morphological locus, covering 2113.3 cM, approximately 42% of the total recombination length of the cotton genome. A total of 31 QTL with 5.1-25.8% of the total phenotypic variance explained were detected. Twenty-four common QTL across environments showed high stability, and six QTL were environment-specific. Several genomic segments affecting multiple traits were identified. The advantage of QTL mapping using a 4WC were discussed. This study presents the first example of QTL mapping using a 4WC population in upland cotton. The results presented here will enhance the understanding of the genetic basis of yield and fiber quality traits and enable further marker-assisted selection in cultivar populations in upland cotton.