DCX- Expressing Cells in the Vicinity of the Hypothalamic Neurogenic Niche: A Comparative Study Between Mouse, Sheep, and Human Tissues
DCX- Expressing Cells in the Vicinity of the Hypothalamic Neurogenic Niche: A Comparative Study Between Mouse, Sheep, and Human Tissues
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DOI：
10.1002/cne.23514
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发表时间：
2014-06-01
影响因子：
2.5
通讯作者：
Migaud, Martine
中科院分区：
文献类型：
--
作者：
Batailler, Martine;Droguerre, Marine;Migaud, Martine
神经干细胞和前体细胞在整个成年期持续存在，并且能够通过改变其增殖和分化来响应许多内源性和外源性信号。而成人神经发生已被广泛研究，在海马结构的齿状回和侧脑室壁附近的脑室下区，我们和其他人最近报道的组成性成人神经发生在其他脑结构，包括下丘脑。在这项研究中，我们使用免疫组化研究的神经母细胞标记物双皮质素（DCX）的表达，并比较其在成年绵羊，小鼠和人类下丘脑组织中的表达模式。我们的研究结果表明，DCX阳性细胞类似于未成熟和发育中的神经元发生在广泛的下丘脑核在所有三个物种，虽然具有不同的分布模式。此外，DCX阳性细胞的形态根据其位置而变化。第三脑室附近的DCX阳性细胞的形态非常不成熟的神经母细胞，圆形，没有过程，而那些位于更深的实质，如在腹内侧核梭形，并表现出双极形态。扩展这一观察，我们发现，在进入腹内侧核的未成熟神经元队列中，一些似乎经历了成熟，如DCX与更成熟神经元标记物的部分共定位所揭示的，人神经元蛋白C和D（HuC/D）。本研究进一步证实了成人下丘脑神经原性龛的存在，并认为下丘脑内可能存在迁移路径。J. Comp.神经元522：1966-1985，2014。(c)2013 Wiley Periodicals，Inc.
Neural stem and precursor cells persist postnatally throughout adulthood and are capable of responding to numerous endogenous and exogenous signals by modifying their proliferation and differentiation. Whereas adult neurogenesis has been extensively studied in the dentate gyrus of the hippocampal formation and in the subventricular zone adjacent to the wall of the lateral ventricles, we and others have recently reported constitutive adult neurogenesis in other brain structures, including the hypothalamus. In this study, we used immunohistochemistry to study the expression of the neuroblast marker doublecortin (DCX), and compared its expression pattern in adult ovine, mouse, and human hypothalamic tissues. Our results indicate that DCX-positive cells resembling immature and developing neurons occur in a wide range of hypothalamic nuclei in all three species, although with different distribution patterns. In addition, the morphology of DCX-positive cells varied depending on their location. DCX-positive cells near the third ventricle had the morphology of very immature neuroblasts, a round shape with no processes, whereas those located deeper in the parenchyma such as in the ventromedial nucleus were fusiform and showed a bipolar morphology. Extending this observation, we showed that among the cohort of immature neurons entering the ventromedial nucleus, some appeared to undergo maturation, as revealed by the partial colocalization of DCX with markers of more mature neurons, e.g., human neuronal protein C and D (HuC/D). This study provides further confirmation of the existence of an adult hypothalamic neurogenic niche and argues for the potential existence of a migratory path within the hypothalamus. J. Comp. Neurol. 522:1966-1985, 2014. (c) 2013 Wiley Periodicals, Inc.