Bioactive carbon dots direct the osteogenic differentiation of human bone marrow mesenchymal stem cells
Bioactive carbon dots direct the osteogenic differentiation of human bone marrow mesenchymal stem cells
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DOI：
10.1016/j.colsurfb.2019.03.035
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发表时间：
2019-07-01
影响因子：
5.8
通讯作者：
Liu, Jianguo
中科院分区：
文献类型：
--
作者：
Han, Yu;Zhang, Fan;Liu, Jianguo
骨髓间充质干细胞（BMSCs）因其良好的成骨潜能和丰富的来源而成为骨再生研究的热点。然而，骨髓间充质干细胞向功能性成骨细胞分化的高成本和低效率限制了其临床应用。希望开发生物活性材料，以生物相容性方式整合有效的分化和可追踪的特性，用于基于MSC的治疗。本研究以腺苷和阿司匹林为原料，采用一步水热法制备了一种新型的生物活性碳点。这些生物活性CD具有细胞相容性和生物安全性，能够长期荧光追踪人骨髓间充质干细胞（hBMSCs）。值得注意的是，生物活性CD的存在通过促进成骨转录和增强基质矿化触发并指导了一系列遵循成骨分化时间模式的事件。此外，具有生物活性CD的细胞比单独用腺苷或阿司匹林处理的细胞表现出更有效的成骨分化行为。总之，这些发现清楚地表明，腺苷和阿司匹林为基础的CD可以直接hBMSCs的成骨分化，在没有任何外部骨诱导因子。生物活性CD的独特性质可以深入了解其实现有效和安全的基于MSC的治疗的潜力。
Bone marrow mesenchymal stem cells (BMSCs) have been the focus of bone regeneration due to their excellent osteogenic potential and abundant source. However, the high-cost and low-efficiency differentiation of BMSCs into functional osteoblasts limits their clinical application. It is desirable to develop bioactive materials to integrate efficient differentiation and traceable properties in a biocompatible manner for MSC-based therapy. In this study, a new kind of bioactive carbon dot (CD) was facilely fabricated through a one-step hydrothermal method from adenosine and aspirin. These bioactive CDs were cytocompatible and biosafe with the capability of long-term fluorescent tracking of human bone marrow mesenchymal stem cells (hBMSCs). Notably, the presence of bioactive CDs triggered and directed a series of events that followed the temporal pattern of osteogenic differentiation through the promotion of osteogenic transcription and enhancement of matrix mineralization. Moreover, cells with bioactive CDs exhibited more effective osteogenic differentiation behavior than cells treated with either adenosine or aspirin alone. Overall, these findings clearly showed that adenosine and aspirin based CDs can direct osteogenic differentiation of hBMSCs in the absence of any external osteoinductive factors. The unique properties of bioactive CDs could provide insight into their potential for achieving efficient and safe MSC-based therapy.