Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells
Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells
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DOI：
10.1089/scd.2007.0077
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发表时间：
2008-02-01
影响因子：
4
通讯作者：
Yamaguchi, Kazunari
中科院分区：
文献类型：
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作者：
Mizukami, Takuo;Kuramitsu, Madoka;Yamaguchi, Kazunari
生殖系干细胞（Germ-line stem cells，GSCs）是一类在体外具有稳定性和增殖能力的干细胞群体，是研究定向组织干细胞自我更新和干细胞功能的有效模型。为了鉴定GSC特异性基因，我们使用GSC、睾丸和胚胎干细胞（ES）的cDNA进行消减杂交，成功鉴定了11个在GSC中高表达的基因。组织学分析证实了Cry alpha B、Mcpt 8、Cxcl 5、Fth 1、Ctla 2 alpha和Spp 1在睾丸生精上皮基底膜区域的未分化精原细胞中的表达，GSC龛被认为位于该区域。在GSC特异性基因中，定量PCR分析显示Fth 1、Cryab、Spp 1、Bcap 31、Arhgap 1、Ctla 2a和Serpina 3g 7个基因是造血干细胞（HSC）中高表达的常见转录本。组织学分析证实，Ctla 2 α-，Serpina 3g-，和Spp 1-表达细胞中观察到的骨小梁区域的骨髓，其中HSC的小生境的位置。此外，组织学分析显示，只有Spp 1在毛囊干细胞龛区域的毛囊隆起中表达。因此，通过与GSC的比较分析来识别干细胞基因是探索HSC和其他干细胞类型的基本共性的有力工具。
Germ-line stem cells (GSCs) constitute a stem cell population with remarkable stability and proliferative potential in vitro and are a useful model for studying the mechanism of self-renewal and "stemness" function of committed tissue stem cells. To identify GSC-specific genes, we performed subtractive hybridization using cDNA from GSCs, testis, and embryonic stem (ES) cells, and successfully identified 11 genes highly expressed in GSCs. Histological analysis confirmed expression of Cry alpha b, Mcpt8, Cxcl5, Fth1, Ctla2 alpha, and Spp1 in undifferentiated spermatogonia on the basement membrane area of the seminiferous epithelium of the testis, where the GSC niche is thought to be located. Among GSC-specific genes, quantitative PCR analysis showed seven genes-Fth1, Cryab, Spp1, Bcap31, Arhgap1, Ctla2a, and Serpina3g-to be common transcripts highly expressed in hematopoietic stem cells (HSCs). Histological analysis confirmed that Ctla2 alpha-, Serpina3g-, and Spp1-expressing cells were observed in the trabecular bone region of the bone marrow, where the HSC niche is located. Furthermore, histological analysis revealed that only Spp1 was expressed in the hair follicle bulge in the area of the hair follicle stem cell niche. Thus, identifying stemness genes by comparative analysis to GSCs is a powerful tool with which to explore the fundamental commonalities of HSCs and other stem cell types.