Okadaic acid stimulates the activity of microtubule associated protein kinase in PC-12 pheochromocytoma cells.
Okadaic acid stimulates the activity of microtubule associated protein kinase in PC-12 pheochromocytoma cells.
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冈田酸刺激 PC-12 嗜铬细胞瘤细胞中微管相关蛋白激酶的活性。
DOI：
10.1016/0006-291x(90)91161-k
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发表时间：
1990
影响因子：
3.1
通讯作者：
Saltiel,AR
中科院分区：
文献类型：
--
作者：
Miyasaka,T;Miyasaka,J;Saltiel,AR
PC-12嗜铬细胞瘤细胞含有磷酸化微管相关蛋白2（MAP-2）的生长因子敏感性蛋白激酶。这种MAP激酶也被蛋白磷酸酶抑制剂冈田酸（OA）激活。此外，OA增强了MAP激酶的NGF依赖性激活，但仅引起EGF观察到的最大激活的适度增强（20%）。由于OA是一种特异的丝氨酸/苏氨酸磷酸酶抑制剂，这些结果表明，丝氨酸/苏氨酸磷酸化可能参与MAP激酶的激素调节。
PC-12 pheochromocytoma cells contain a growth factor-sensitive protein kinase that phosphorylates microtubule associated protein 2 (MAP-2). This MAP kinase is also activated by the protein phosphatase inhibitor okadaic acid (OA). Additionally, OA potentiates the NGF-dependent activation of MAP kinase, but causes only a modest potentiation (20%) of the maximal activation observed with EGF. Since OA is a specific serine/threonine phosphatase inhibitor, these results suggest that serine/threonine phosphorylation may be involved in the hormonal regulation of MAP kinase.