DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli
DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli
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DOI：
10.1128/jb.183.18.5239-5247.2001
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发表时间：
2001-09-01
影响因子：
3.2
通讯作者：
Bentley, WE
中科院分区：
文献类型：
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作者：
DeLisa, MP;Wu, CF;Bentley, WE
细菌细胞间的通讯促进特定基因以生长速率II和细胞密度依赖的方式协调表达，这一过程称为群体感应。虽然已经发现了一种可扩散的大肠杆菌信号信息素（称为自诱导剂 2 (AI-2)）以及一些群体感应基因，但通过 AI-2 信号受群体感应控制的基因的总数和协调尚未得到系统研究。我们通过使用无法合成 AI-2 的 luxS 突变体，研究了 AI-2 信号分子引起的 mRNA 丰度的整体变化。值得注意的是，有 242 个基因，包括大约 1 个基因。 5.6% 的大肠杆菌基因组响应 300 倍的 AI-2 信号差异而表现出显着的转录变化（诱导或抑制），其中许多已识别的基因表现出高诱导水平（超过五倍）。 ygeV（一种假定的 sigma (54) 依赖性转录激活剂）和 yhbH（一种 sigma (54) 调节蛋白）的显着诱导表明 sigma (54) 可能参与大肠杆菌群体感应。
Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density- dependent manner, a process known as quorum sensing. While the discovery of a diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several quorum sensing genes, the overall number and coordination of genes controlled by quorum sensing through the AI-2 signal has not been studied systematically. We investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesize AI-2. Remarkably, 242 genes, comprising ca. 5.6% of the E. coli genome, exhibited significant transcriptional changes (either induction or repression) in response to a 300-fold AI-2 signaling differential, with many of the identified genes displaying high induction levels (more than fivefold). Significant induction of ygeV, a putative sigma (54)-dependent transcriptional activator, and yhbH, a sigma (54) modulating protein, suggests sigma (54) may be involved in E. coli quorum sensing.