Interaction between embryos and culture conditions during in vitro development of bovine early embryos
Interaction between embryos and culture conditions during in vitro development of bovine early embryos
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DOI：
10.1017/s0967199408004644
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发表时间：
2008-05-01
期刊：
影响因子：
1.7
通讯作者：
Saeki, Kazuhiro
中科院分区：
文献类型：
--
作者：
Nagao, Yoshikazu;Iijima, Rumi;Saeki, Kazuhiro
胚胎密度和培养液中的物质等多种因素都会影响胚胎的体外发育。这些因素和胚胎可能相互作用，但相互作用尚未完全了解。为了研究这种相互作用，我们检查了牛IVM/IVF胚胎无蛋白培养期间胚胎数量、液滴大小、氧气浓度以及培养基中葡萄糖和无机磷酸盐的影响。在实验1中，将不同数量的胚胎培养在50 μ l的培养基中。25、50和100个胚胎/滴组的囊胚发育率高于其他组。将1个、5个和25个胚胎分别培养在不同大小的液滴中（实验2）、50 μ l不同O2浓度的培养基中（实验3）和50 μ l不含葡萄糖和/或无机磷酸盐的培养基中（实验4）。在实验2中，培养基液滴的大小没有改善囊胚发育。在实验3中，囊胚发育的最高频率为1，5和25个胚胎/滴分别在1，2.5和5%O（2），。在实验4中，在不含无机磷酸盐的培养基中，每滴1个和5个胚胎的囊胚发育得到改善。这些结果表明，在培养过程中，胚胎之间存在着合作的相互作用，这种相互作用可能是通过减少培养基中的毒性因子来介导的。在低胚胎密度下，降低氧浓度或排除无机磷酸盐促进囊胚发育。
Various factors such as embryo density and substances in the medium can influence embryo development in vitro. These factors and the embryos probably interact with each other, however the interactions are not fully understood. To invetigate the interactions, we examined the effects of the number of embryos, drop size, oxygen concentration and glucose and inorganic phosphate in the medium during protein-free culture of bovine IVM/IVF embryos. In Experiment 1, different numbers of embryos were cultured in a 50 mu l drop of medium. The frequencies of blastocyst development in the groups with 25, 50 and 100 embryos per drop were higher than in the other groups. One, five and 25 embryos were cultured in different drop sizes (Experiment 2), a 50 mu l drop of medium at different O(2) concentrations (Experiment 3) and a 50 mu l drop of medium excluding glucose and/or inorganic phosphate (Experiment 4). In Experiment 2, the size of the medium drops did not improve blastocyst development. In Experiment 3, the highest frequency of blastocyst development for one, five and 25 embryos per drop was obtained at 1, 2.5 and 5% O(2), respectively. In Experiment 4, blastocyst development for one and five embryos per drop were improved in the medium excluded inorganic phosphate. These results indicate that there is a cooperative interaction among embryos during culture and that this interaction may be mediated by reduction of toxic factors in the medium. At low embryo density, reduced oxygen concentration or the exclusion of inorganic phosphate enhanced blastocyst development.