EphB receptors interact with NMDA receptors and regulate excitatory synapse formation
EphB receptors interact with NMDA receptors and regulate excitatory synapse formation
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DOI：
10.1016/s0092-8674(00)00197-5
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发表时间：
2000-12-08
期刊：
影响因子：
64.5
通讯作者：
Greenberg, ME
中科院分区：
文献类型：
--
作者：
Dalva, MB;Takasu, MA;Greenberg, ME
突触处富含EphB受体酪氨酸激酶，提示这些受体在突触的形成或功能中发挥作用。我们发现，EphB与EphB结合可诱导EphB与NMDA型谷氨酸受体直接相互作用。这种相互作用发生在细胞表面，并由两个受体的胞外区介导，但不需要EphB的激酶活性。EphB的激酶活性对于突触形成的后续步骤可能是重要的，因为EphB酪氨酸激酶活性的扰动会影响培养神经元中形成的突触特化的数量。这些发现表明，EphB激活EphB促进了EphB与NMDA受体的联系，这可能是突触发育或功能的关键。
EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. The kinase activity of EphB may be important for subsequent steps in synapse formation, as perturbation of EphB tyrosine kinase activity affects the number of synaptic specializations that form in cultured neurons. These findings indicate that EphrinB activation of EphB promotes an association of EphB with NMDA receptors that may be critical for synapse development or function.