Expression of SPANX proteins in human-ejaculated spermatozoa and sperm precursors
Expression of SPANX proteins in human-ejaculated spermatozoa and sperm precursors
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DOI：
10.1111/j.1365-2605.2004.00461.x
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发表时间：
2004-06-01
影响因子：
--
通讯作者：
Vicari, E
中科院分区：
文献类型：
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作者：
Salemi, M;Calogero, AE;Vicari, E
X染色体（SPAN）基因家族中与细胞核相关的精子蛋白仅由少数成员组成，聚集在Xq27，编码15至20 kDa的小蛋白。这些蛋白质已被证明存在于成熟精子和肿瘤中，如黑色素瘤和一些白血病。我们开发了多克隆血清，以研究该蛋白在人类射精精子及其前体中的分布。从SPAN蛋白家族共有的结构域设计合成肽，并在小鼠中产生多克隆血清。招募7名精子参数正常的健康志愿者，通过免疫细胞化学和免疫荧光分析评估精子和射出精子前体中的SPAN X蛋白表达。在成熟精子中，存在大部分（96%）的SPAN蛋白质，其定位于精子头部（39.2%）、中段（22.8%）或两个部位（34.4%）。精子细胞也表现出存在的斯潘X蛋白在其细胞质中，虽然显着较高数量的精子细胞与精子相比，斯潘X阴性。总之，SPAN蛋白在精子细胞和成熟精子中表达的百分比升高。在后者中，它们优先位于精子头部。观察到的大量的SPAN阴性精子细胞可能与它们更容易从曲细精管脱落有关。
The sperm protein associated with nucleus in the X chromosome (SPANX) gene family is constituted by only a few members, clustered at Xq27, encoding small proteins which range from 15 to 20 kDa. These proteins have been shown to be present both in mature spermatozoa and in tumours, such as melanoma and some leukaemias. We developed polyclonal sera in order to study the distribution of the protein in human-ejaculated spermiatozoa and their precursors. A synthetic peptide was designed from a domain common to the SPANX protein family and polyclonal sera were raised in mice. Seven healthy volunteer men with normal sperm parameters were recruited and the expression of SPANX proteins was evaluated in spermatozoa and ejaculated sperm precursors by immunocytochemistry and immunofluorescence analyses. SPANX proteins, present in a large fraction (96%) of mature spermatozoa, were localized in the sperm head (39.2%), midpiece (22.8%) or in both sites (34.4%). Spermatids also showed the presence of SPANX proteins in their cytoplasm, although a significantly higher number of spermatids were SPANX-negative compared with spermatozoa. In conclusion, SPANX proteins are expressed in an elevated percentage of spermatids and mature spermatozoa. In the latter, they are preferentially located in the sperm head. The greater number of SPANX-negative spermatids observed could relate to their easier exfoliation from the seminiferous tubules.