Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification
Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification
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DOI：
10.1016/j.vaccine.2014.04.033
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发表时间：
2014-06-24
期刊：
影响因子：
5.5
通讯作者：
Lundgren, Mats
中科院分区：
文献类型：
--
作者：
Blom, Hans;Akerblom, Anna;Lundgren, Mats
接种疫苗是避免流感感染和保护大量人口的最有效的预防策略。绝大多数流感疫苗仍然是用受精鸡蛋的尿囊液生产的。卵清蛋白占尿囊液总蛋白含量的60%以上，可导致严重过敏。因此，在以鸡蛋为基础的疫苗生产过程中，有效降低卵清蛋白是至关重要的。在这里，我们介绍了Capto Core 700，一种通过模式树脂流动的新型核心珠层析去除卵蛋白，并将其与蔗糖带状梯度超速离心法进行比较，蔗糖带状梯度超速离心法是鸡蛋疫苗生产的行业标准。结果表明，核心珠层析法在去除卵清蛋白方面完全可以与区带离心法相媲美，满足法规要求。此外，与分区离心法相比，这种方法的可扩展性和较短的处理时间有可能显著提高工业生产的生产率和经济性。(C)2014爱思唯尔有限公司。保留所有权利。
Vaccination is the most effective prevention strategy to avoid influenza infection and for protection of large populations. The vast majority of influenza vaccines are still produced with allantoic fluid from fertilized chicken eggs. The presence of ovalbumin, which can constitute over 60% of the total protein content in allantoic fluid, can result in severe allergies. Consequently, efficient reduction of ovalbumin is critical during egg based vaccine manufacturing. Here we present Capto Core 700, a novel core bead chromatographic flow through mode resin for removal of ovalbumin and compare it to sucrose zonal gradient ultracentrifugation, which is the industry standard for egg-based vaccine production. The results demonstrate that core bead chromatography is fully comparable to zonal centrifugation in removing ovalbumin to meet regulatory requirements. Furthermore, the scalability and the shorter process times of this method have the potential to significantly improve the productivity and economy for industrial production compared to zonal centrifugation. (C) 2014 Elsevier Ltd. All rights reserved.