A MOUSE MONOCLONAL-ANTIBODY DETECTING A DR-RELATED MT2-LIKE SPECIFICITY - SEROLOGY AND BIOCHEMISTRY
A MOUSE MONOCLONAL-ANTIBODY DETECTING A DR-RELATED MT2-LIKE SPECIFICITY - SEROLOGY AND BIOCHEMISTRY
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DOI：
10.1016/0198-8859(84)90027-2
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发表时间：
1984-01-01
期刊：
影响因子：
2.7
通讯作者：
BRUNING, H
中科院分区：
文献类型：
--
作者：
KONING, F;SCHREUDER, I;BRUNING, H
制备了一种仅与B细胞和单核细胞DR3、DR5和/或DLu6阳性供体表面的II类分子发生反应的小鼠单抗[Moab](7.3.19.1)。利用这个摩押和其他两个摩押，可以鉴定出3个不同的II类分子基团。发现一种差异沉淀模式，它与细胞表面MT2样多态决定簇的DR相关变量表达相关。在MLC[混合淋巴细胞培养]中加入7.3.19.1与另外两个受试的单抗相比，没有引起明显的抑制作用。在这样一个刺激反应者群体中发现了正常的CTL[细胞毒性T淋巴细胞]活性。
A mouse monoclonal antibody [Moab] (7.3.19.1) was produced which reacts with class II molecules on B cells and monocytes DR3, DR5 and/or DRw6 positive donors only. Using this Moab and 2 others, 3 different groups of class II molecules could be identified. A differential precipitation pattern was found which correlates with a DR-related variable expression of the MT2-like polymorphic determinant on the cell surface. Addition of 7.3.19.1 to MLC [mixed lymphocyte culture] did not result in significant inhibition in contrast to the 2 other Moab tested. Normal CTL [cytotoxic T lymphocyte] activity was found in such a stimulated responder population.