Bidirectional transport by distinct populations of COPI-coated vesicles
Bidirectional transport by distinct populations of COPI-coated vesicles
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DOI：
10.1016/s0092-8674(00)80341-4
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发表时间：
1997-07-25
期刊：
影响因子：
64.5
通讯作者：
Rothman, JE
中科院分区：
文献类型：
--
作者：
Orci, L;Stamnes, M;Rothman, JE
电子显微镜免疫细胞化学显示，顺行定向（胰岛素原和VSV G蛋白）和逆行定向（KDEL受体）货物存在于从全细胞中高尔基体堆叠的每个水平出芽的coPI包被的囊泡中;然而，它们包含两个不同的群体，这两个群体一起可以占从高尔基体池出芽的囊泡的至少80%。分离的顺级从逆行定向货物到不同的COPI包被的囊泡组中忠实地再现在无细胞高尔基体转运系统中，其中VSV G蛋白和KDEL受体被包装到可分离的囊泡中，即使当出芽是由高度纯化的coatomer和重组ARF蛋白驱动时。
Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in coPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of antero-grade- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.