Origins of inner ear sensory organs revealed by fate map and time-lapse analyses
Origins of inner ear sensory organs revealed by fate map and time-lapse analyses
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DOI：
10.1006/dbio.2001.0211
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发表时间：
2001-05-15
影响因子：
2.7
通讯作者：
Collazo, A
中科院分区：
文献类型：
--
作者：
Kil, SH;Collazo, A
内耳从一个简单的外胚层增厚的耳胎板发育成一个迷宫般的房间，里面有感觉器官，用来感知声音并用来保持平衡。虽然感觉器官的形态和功能有了很好的描述，但它们的起源和血统关系实际上是未知的。在这项研究中，我们制作了非洲爪哇内耳在耳道胎盘和耳囊阶段的命运图，以确定感觉器官的发育起源。我们的谱系分析表明，耳盘和耳囊的所有区域都可以产生内耳的感觉器官，尽管标记象限之间形成的导数范围不同。一个给定的区域通常在多个感觉器官中产生细胞，包括明显从前极到后极散布的神经元，反之亦然。这些结果表明，单个感觉器官来自胎盘或耳囊不同部分的细胞，细胞混合在耳朵发育中起着重要作用。延时视频显微镜提供了进一步的证据，来自内耳相反区域的细胞在内耳的发育过程中混合，这种混合在胎盘阶段开始。最后，骨形态发生蛋白4(BMP-4)是转化生长因子-β(TGF-β)家族的成员之一，在蛙内耳的所有感觉器官中都有表达，在发育中的鸡耳中也有表达。内耳命运图为解释基因表达模式和胚胎学操作提供了背景。(C)2001年学术出版社。
The inner ear develops from a simple ectodermal thickening called the otic placode into a labyrinth of chambers which house sensory organs that sense sound and are used to maintain balance. Although the morphology and function of the sensory organs are well characterized, their origins and lineage relationships are virtually unknown. In this study, we generated a fate map of Xenopus laevis inner ear at otic placode and otocyst stages to determine the developmental origins of the sensory organs. Our lineage analysis shows that all regions of the otic placode and otocyst can give rise to the sensory organs of the inner ear, though there were differences between labeled quadrants in the range of derivatives formed. A given region often gives rise to cells in multiple sensory organs, including tells that apparently dispersed from anterior to posterior poles and vice versa. These results suggest that a single sensory organ arises from cells in different parts of the placode or otocyst and that cell mixing plays a large role in ear development. Time-lapse videomicroscopy provides further evidence that cells from opposite regions of the inner ear mix during the development of the inner ear, and this mixing begins at placode stages. Lastly, bone morphogenetic protein 4 (BMP-4), a member of the transforming growth factor beta (TGF-beta) family, is expressed in all sensory organs of the frog inner ear, as it is in the developing chicken ear. Inner ear fate maps provide a context for interpreting gene expression patterns and embryological manipulations. (C) 2001 Academic Press.