RECA-INDEPENDENT AND RECA-DEPENDENT INTRAMOLECULAR PLASMID RECOMBINATION - DIFFERENTIAL HOMOLOGY REQUIREMENT AND DISTANCE EFFECT
RECA-INDEPENDENT AND RECA-DEPENDENT INTRAMOLECULAR PLASMID RECOMBINATION - DIFFERENTIAL HOMOLOGY REQUIREMENT AND DISTANCE EFFECT
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DOI：
10.1006/jmbi.1994.1002
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发表时间：
1994-01-14
影响因子：
5.6
通讯作者：
LIU, LF
中科院分区：
文献类型：
--
作者：
BI, X;LIU, LF
我们发现，在大肠杆菌中，质粒中直接重复序列之间的重组发生在reca独立和reca依赖的机制下。它们受到两个因素的不同影响，即同源物之间的距离和同源物的大小。高达300个碱基对的串联复制之间的重组显示几乎没有依赖性。增加超过300个碱基对的复制的大小逐渐增加了它们的依赖性。此外，在复制之间插入相当大的DNA序列，大大增加了其依赖性。我们得出结论，增加同源区域之间的距离会优先抑制不依赖于eca的重组。另一方面，同源性的缩短优先抑制reca依赖性重组。因此。短串联重复之间的重组是完全不依赖于基因的。
We show that recombination between directly repeated sequences in plasmids occursviabothrecA-independent andrecA-dependent mechanisms inEscherichia coli. They are differentially affected by two factors, the distance separating the homologies and the size of the homology. Recombination between tandem duplications up to 300 base-pairs shows virtually norecAdependence. Increasing the size of the duplications beyond 300 base-pairs gradually increases therecAdependence. Furthermore, insertion of a sizable DNA sequence in between the duplications, substantially increases therecAdependence. We conclude that increasing the distance separating the homologous regions preferentially inhibits therecA-independent recombination. On the other hand, shortening of the homology preferentially inhibitsrecA-dependent recombination. Consequently. recombination between short tandem duplications is totallyrecA-independent.