Combined overexpression of HIVEP3 and SOX9 predicts unfavorable biochemical recurrence-free survival in patients with prostate cancer
Combined overexpression of HIVEP3 and SOX9 predicts unfavorable biochemical recurrence-free survival in patients with prostate cancer
复制标题
HIVEP3和SOX9的联合过表达可预测前列腺癌患者不利的生化无复发生存
DOI：
10.2147/ott.s55432
复制
发表时间：
2014-01-01
影响因子：
4
通讯作者：
Zhong, Wei-de
中科院分区：
文献类型：
--
作者：
Qin, Guo-qiang;He, Hui-chan;Zhong, Wei-de
背景资料：目的：探讨HIV-3和SOX-9共表达在前列腺癌（PCa）中的作用。方法：采用小分子干扰RNA（siRNA）技术，在PCa细胞中抑制SOX-9的表达，并在体外分析SOX-9抑制剂对HIV-3表达的影响。结果：下调SOX 9基因表达可抑制体外培养的PCa细胞中HIV-3的表达。此外，PCa组织中HIVEP 3和SOX 9 mRNA的表达水平均显著高于非癌前列腺组织（P=0.006和P
Background: To clarify the involvement of HIVEP3 and SOX9 coexpression in prostate cancer (PCa).Methods: A small interfering RNA was used to knockdown SOX9 expression in a PCa cell line and to analyze the effects of SOX9 inhibition on the expression of HIVEP3 in vitro. Then, HIVEP3 and SOX9 expression patterns in the human PCa tissues were detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis and immunohistochemistry.Results: We found that the downregulation of SOX9 could inhibit the expression of HIVEP3 in the PCa cells in vitro. In addition, both HIVEP3 and SOX9 messenger RNA expression levels in the PCa tissues were significantly higher than those in the noncancerous prostate tissues (P=0.006 and P