Fine mapping identifies CsGCN5 encoding a histone acetyltransferase as putative candidate gene for tendril-less1 mutation (td-1) in cucumber
Fine mapping identifies CsGCN5 encoding a histone acetyltransferase as putative candidate gene for tendril-less1 mutation (td-1) in cucumber
复制标题
精细定位将编码组蛋白乙酰转移酶的 CsGCN5 确定为黄瓜中 tentril-less1 突变 (td-1) 的假定候选基因
DOI：
10.1007/s00122-017-2909-1
复制
发表时间：
2017-08-01
影响因子：
5.4
通讯作者：
Li, Yuhong
中科院分区：
文献类型：
--
作者：
Chen, Feifan;Fu, Bingbing;Li, Yuhong
下一代测序辅助图谱克隆技术将黄瓜卷茎- less1 （td - 1）位点定位到6号染色体190.7 kb的区域，该区域含有一个推测的新功能候选基因，编码组蛋白乙酰转移酶（CsGCN5）。卷须起源于侧分生组织，是葫芦科包括黄瓜（Cucumis sativus L.）在内的物种的重要器官。卷须在具有进化意义的同时，也给保护性环境下的黄瓜栽培带来了困扰，而无卷须黄瓜在保护性环境下有其优势。从一个EMS突变群体中，我们发现了一个由隐性基因td-1控制的无卷须突变体B007。通过下一代测序辅助图谱克隆，我们发现黄瓜组蛋白乙酰转移酶基因CsGCN5 （Cucumis sativus GENERAL CONTROL NONDEREPRESSIBLE 5）是td-1最可能的候选基因。CsGCN5第一个外显子的非同义SNP导致野生型的Asp (D)氨基酸替换为无卷须突变体的Asn (N)氨基酸。在双亲本和天然黄瓜群体中，多重证据进一步证实了CsGCN5的候选性。在野生型和突变型之间，CsGCN5在多个器官中无显著表达。CsGCN5在野生型植物卷须中有较强的表达，提示其在植物卷须的生长发育中起重要作用。本研究的td-1突变体的鉴定和鉴定为了解黄瓜卷须器官发生的分子机制和研究组蛋白乙酰转移酶的新功能提供了有用的工具。
Next-generation sequencing-aided map-based cloning delimited the cucumber tendril - less1 ( td - 1 ) locus into a 190.7-kb region in chromosome 6 harboring a putative, novel-function candidate gene encoding a histone acetyltransferase ( CsGCN5 ). The tendril initiated from the lateral meristem is an important and characteristic organ for the species in the Cucurbitaceae family including cucumber (Cucumis sativus L.). While the tendril has its evolutionary significance, it also poses a nuisance in cucumber cultivation under protected environments in which tendril-less cucumber has its advantages. From an EMS mutagenesis population, we identified a tendril-less mutant B007, which was controlled by a recessive gene td-1. Through next-generation sequencing-aided map-based cloning, we show CsGCN5 (Cucumis sativus GENERAL CONTROL NONDEREPRESSIBLE 5), a cucumber gene for a histone acetyltransferase as the most possible candidate for td-1. A non-synonymous SNP in the first exon of CsGCN5 resulted in an amino-acid substitution from Asp (D) in the wild type to Asn (N) in the tendril-less mutant. The candidacy of CsGCN5 was further confirmed by multiple lines of evidence in both biparental and natural cucumber populations. Non-significant expression of CsGCN5 in multiple organs was found between the wild type and the mutant. CsGCN5 exhibited strong expression in the tendril of wild-type plants suggesting its important roles in growth and development of plant tendrils. The identification and characterization of the td-1 mutant from the present study provided a useful tool in understanding the molecular mechanisms of tendril organogenesis and investigation of novel functions of the histone acetyltransferase in cucumber.