Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones.
Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones.
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在休斯顿发现的耐青霉素肺炎链球菌菌株：多个克隆的鉴定和分子特征。
DOI：
10.1093/infdis/167.4.850
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Musser,JM
中科院分区：
文献类型：
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作者：
Versalovic,J;Kapur,V;MasonJr,EO;Shah,U;Koeuth,T;Lupski,JR;Musser,JM
1989年1月至1991年5月，主要从休斯顿感染儿童中回收了48株青霉素耐药肺炎链球菌（PRSP）菌株，采用多位点酶电泳和重复基因外回文聚合酶链反应基因组分析进行了表征。鉴定了22种克隆基因型的异质阵列，但样品中64%的PRSP菌株被分配到5个克隆，这些克隆在总体染色体特征上非常相似，并表达血清型6荚膜。多位点酶电泳鉴定了这5个克隆与在阿拉斯加、冰岛和西班牙回收的青霉素耐药6B克隆之间的密切遗传关联。总之，这些结果表明，阿拉斯加、冰岛、西班牙和休斯顿的常见耐药6B克隆有一个最近的祖先，或者某种肺炎球菌系统发育谱系的分离株更有可能产生青霉素耐药性。
A sample of 48 penicillin-resistantStreptococcus pneumoniae(PRSP) strains recovered between January 1989 and May 1991, primarily from infected children in Houston, was characterized by multilocus enzyme electrophoresis and repetitive extragenic palindromic-polymerase chain reaction genomic profiling. A heterogeneous array of 22 clonal genotypes was identified, but 64% of the PRSP strains in the sample were assigned to five clones that are closely similar in overall chromosomal character and express serotype 6 capsule. A close genetic association between these five clones and penicillin-resistant 6B clones recovered in Alaska, Iceland, and Spain was identified by multilocus enzyme electrophoresis. Taken together, the results suggest either that the common resistant 6B clones in Alaska, Iceland, Spain, and Houston have a recent ancestor or that isolates of a certain pneumococcal phylogenetic lineage are more likely to develop penicillin resistance.