Two deltaC splice-variants have distinct signaling abilities during somitogenesis and midline patterning.
Two deltaC splice-variants have distinct signaling abilities during somitogenesis and midline patterning.
复制标题
两个 deltaC 剪接变体在体节发生和中线模式形成过程中具有不同的信号传导能力。
DOI：
10.1016/j.ydbio.2008.03.009
复制
发表时间：
2008
影响因子：
2.7
通讯作者：
Holley,ScottA
中科院分区：
文献类型：
--
作者：
Mara,Andrew;Schroeder,Joshua;Holley,ScottA
Notch signaling is required for many developmental processes, yet differences in the signaling abilities of various Notch ligands are poorly understood. Here, we have isolated a splice variant of the zebrafish Notch ligand deltaC in which the inclusion of the last intron leads to a truncation of the C-terminal 39 amino acids (deltaCtv2). We show that, unlike deltaCtv1, deltaCtv2cannot function effectively in somitogenesis but has an enhanced ability to signal during midline development. Additionally, over-expression of deltaCtv2preferentially affects anterior midline development, while another Notch ligand, deltaD, shows a posterior bias. Using chimeric Deltas we show that the intracellular domain is responsible for the strength of signal in midline development, while the extracellular domain influences the anterior–posterior bias of the effect. Together our data show that different deltas can signal in biologically distinct ways in both midline formation and somitogenesis. Moreover, it illustrates the importance of cell-type-dependent modifiers of Notch signaling in providing ligand specificity.
