Comparative analysis of the mitochondrial DNA control region in cyprinids with different ploidy level
Comparative analysis of the mitochondrial DNA control region in cyprinids with different ploidy level
复制标题
DOI：
10.1016/s0044-8486(03)00168-6
复制
发表时间：
2003-06-30
期刊：
影响因子：
4.5
通讯作者：
Liu, Y
中科院分区：
文献类型：
--
作者：
Guo, XH;Liu, SJ;Liu, Y
对不同倍性水平的鲤科鱼线粒体DNA全控制区（mtDNA-CR）序列进行了综合分析。采用聚合酶链反应（PCR）扩增异体三倍体鲫鱼和异体三倍体鲤鱼的mtDNA-CR序列，并对扩增产物进行测序。比较分析了红鲫、鲤鱼、鲫鱼、斑马鱼等二倍体与异体四倍体、异体三倍体、异体三倍体鲤等多倍体的mtDNA-CR序列。观察了7种鲤科鱼类mtDNA-CR的延伸末端相关序列（ETAS）、中心保守序列片段（CSB-F、CSB-E、CSB-D）、保守序列片段（CSB-1、CSB-2、CSB-3）和可能的启动子。我们的数据与哺乳动物mtDNA-CR中的三个结构域（扩展末端相关序列，中心，保守序列块结构域）的观点一致。mtDNA-CR中最保守的区域是中央保守序列块结构域（34.06%的核苷酸位置可变），而ETAS和CSB结构域分别有66.91%和40.00%的核苷酸位置可变。通过对不同倍性水平鲤科动物mtDNA-CR的比较分析，确定了鲤科动物mtDNA-CR功能保守单元的一致序列。这些结果为进一步研究鱼类mtDNA-CR结构提供了有用的重要信息。同种异体三倍体鲫鱼和异体三倍体鲤鱼与其母本的mtDNA-CR序列相似性高于同种异体三倍体鲫鱼和同种异体三倍体普通鲤鱼与其父本的mtDNA-CR序列相似性，为同种异体三倍体鲫鱼和同种异体三倍体普通鲤鱼mtDNA-CR的母性遗传提供了直接证据。(C) 2003 Elsevier Science b.v.版权所有
The comprehensive analysis of the complete mitochondrial DNA control region (mtDNA-CR) sequences from the different ploidy level cyprinid fish was made. The mtDNA-CR sequences for allotriploid crucian carp and allotriploid common carp were amplified by the polymerase chain reaction (PCR) and the amplified products were sequenced. The mtDNA-CR sequences from diploids, such as the red crucian carp, common carp, Japanese crucian carp, and zebrafish to polyploids, such as the allotetraploid, allotriploid crucian carp, and allotriploid common carp, were compared and analyzed. The extended terminal associated sequences (ETAS), central conserved sequence blocks (CSB-F, CSB-E, CSB-D), conserved sequence blocks (CSB-1, CSB-2, CSB-3), and putative promoters of the mtDNA-CR in the seven cyprinid fish were observed. Our data were consistent with the opinion of three domains (extended terminal associated sequences, central, conserved sequence block domains) within the mtDNA-CR in the mammals. The most conserved region in the mtDNA-CR was the central conserved sequence block domain (34.06% of nucleotide positions variable), whereas in the ETAS and CSB domains, 66.91 % and 40.00% of nucleotide positions were variable, respectively. Based on the comparative analysis of the mtDNA-CR in cyprinids with different ploidy level, the consensus sequences of functional conserved units of the mtDNA-CR in cyprinids were identified. These results provided the useful and important information for the further study of the mtDNA-CR structure in fish. The similarities of the mtDNA-CR sequences between the allotriploid crucian carp and allotriploid common carp and their female parents were higher than those between allotriploid crucian carp and allotriploid common carp and their male parents, providing the direct evidence of the maternal inheritance of the mtDNA-CR in the allotriploid crucian carp and allotriploid common carp. (C) 2003 Elsevier Science B.V All rights reserved.