The Switchgrass Genome: Tools and Strategies
The Switchgrass Genome: Tools and Strategies
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DOI：
10.3835/plantgenome2011.10.0026
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发表时间：
2011-11-01
期刊：
影响因子：
4.2
通讯作者：
Ronald, Pamela
中科院分区：
文献类型：
--
作者：
Casler, Michael D.;Tobias, Christian M.;Ronald, Pamela
柳枝稷是一种多年生禾本科植物，作为潜在的生物能源作物受到广泛关注。在过去的5年里，柳枝稷研究社区已经产生了一个遗传连锁图，一个表达序列标签（EST）数据库，一组分布在18个连锁群中的单核苷酸多态性（SNP）标记，在400-bp读取中的P. virgatum AP 13基因组的4倍采样，以及包含超过200，000个克隆的细菌人工染色体（BAC）文库。这些研究揭示了柳枝稷基因组与高粱基因组的密切共线性[Sorghum bicolor（L.）Moench]、稻（Oryza sativa L.）、和二穗短柄草Brachypodium distachyon（L.）P. Beauv。柳枝稷研究人员还开发了几种用于基因表达研究的微阵列技术。柳枝稷基因组资源将加速植物育种者提高生产力、抗虫性和营养质量的能力。由于柳枝稷是基因组学世界的一个相对较新的物种，柳枝稷基因组的许多秘密尚未被揭示。为了继续有效地探索柳枝稷的基本和应用主题，在开发和利用该物种基因组资源的下一个逻辑步骤中，捕获和利用植物遗传学家和育种家的知识将是至关重要的。为此，社区成立了柳枝稷基因组学执行委员会和工作组（http：//switchgrassgenomics.org/[2011年10月28日核实]）。
Switchgrass (Panicum virgatum L.) is a perennial grass species receiving significant focus as a potential bioenergy crop. In the last 5 yr the switchgrass research community has produced a genetic linkage map, an expressed sequence tag (EST) database, a set of single nucleotide polymorphism (SNP) markers that are distributed across the 18 linkage groups, 4x sampling of the P. virgatum AP13 genome in 400-bp reads, and bacterial artificial chromosome (BAC) libraries containing over 200,000 clones. These studies have revealed close collinearity of the switchgrass genome with those of sorghum [Sorghum bicolor (L.) Moench], rice (Oryza sativa L.), and Brachypodium distachyon (L.) P. Beauv. Switchgrass researchers have also developed several microarray technologies for gene expression studies. Switchgrass genomic resources will accelerate the ability of plant breeders to enhance productivity, pest resistance, and nutritional quality. Because switchgrass is a relative newcomer to the genomics world, many secrets of the switchgrass genome have yet to be revealed. To continue to efficiently explore basic and applied topics in switchgrass, it will be critical to capture and exploit the knowledge of plant geneticists and breeders on the next logical steps in the development and utilization of genomic resources for this species. To this end, the community has established a switchgrass genomics executive committee and work group (http://switchgrassgenomics.org/[verified 28 Oct. 2011]).