Application of Electrohydrodynamic Technology for Folic Acid Encapsulation
Application of Electrohydrodynamic Technology for Folic Acid Encapsulation
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DOI：
10.1007/s11947-012-0843-4
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发表时间：
2013-07-01
影响因子：
5.6
通讯作者：
Edirisinghe, Mohan
中科院分区：
文献类型：
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作者：
Bakhshi, Poonam Kaushik;Nangrejo, M. Rafique;Edirisinghe, Mohan
叶酸是一组对中枢神经系统生长和发育至关重要的维生素。这些叶酸的天然衍生物大多容易氧化，对热、温度、氧气和光非常敏感。将叶酸包裹在聚合物的惰性基质中可以提高其稳定性，并阻止其被光和氧气降解。电流体动力学（EHD）技术能够根据流速和施加的电势差，从射流的破裂中产生直径从微米到纳米的微小液滴。本研究的目的是利用EHD技术制备包裹在海藻酸钠（Na alginate）中的纳米级叶酸颗粒，并研究电压和流速对颗粒大小以及所制备颗粒结构的影响。已确定在单射流EHD技术中可以使用40mg/ml（海藻酸钠）的浓度。然而，只有10mg/ml的浓度在任何施加的电压和流速下都能提供稳定的射流。因此，采用该浓度并用于包裹更高剂量的叶酸。观察到在12kV电压下，获得均匀直径（4.2±1.2μm）球形颗粒的最佳流速为10μl/min。干燥后，这些颗粒的直径在50 - 200nm范围内，形状变得不太规则。随着叶酸浓度从1mg/ml增加到10mg/ml，在海藻酸钠浓度恒定的情况下，颗粒的产率百分比增加了10%以上，相应的包封效率提高了一倍。傅里叶变换红外光谱（FTIR）研究表明海藻酸钠基质中存在叶酸，并且它们之间没有特征性的化学相互作用。从上述研究结果可以得出结论，在海藻酸钠浓度为10mg/ml、流速为10μl/min和电压为12kV的条件下，大量的叶酸（5mg/ml）可以被包裹在海藻酸钠基质中，具有较高的产率百分比（70%）和负载量（96%），产生直径为90 - 150nm的非球形干燥珠粒/颗粒。
Folates are a group of vitamins vital for the growth and development of the central nervous system. Most of these natural derivatives of folic acid are prone to oxidation and are very sensitive towards heat, temperature, oxygen, and light. Encapsulation of folic acid within inert matrices of a polymer can improve its stability and stop its degradation by light and oxygen. Electrohydrodynamic (EHD) technology is capable of generating fine droplets ranging from micrometers to nanometers in diameter from the breakup of a jet depending on the flow rate and applied electrical potential difference. The aims of this study were to generate nano-sized particles of folic acid encapsulated in sodium alginate (Na alginate) using EHD technology and to study the effect of voltage and flow rate on particle size as well as the structure of the prepared particles. It was established that 40 mg/ml (Na alginate) concentration can be used in single jet EHD technology. However, only 10 mg/ml concentration furnished stable jetting at any applied voltage and flow rate. So, this concentration was utilized and used to encapsulate higher dosages of folic acid. It was observed that the optimum flow rate for obtaining spherical particles of uniform diameter (4.2 +/- 1.2 mu m) was 10 mu l/min at a voltage of 12 kV. Upon drying, these particles acquired a diameter in the range of 50-200 nm and became less spherical in shape. As the folic acid concentration is increased from 1 to 10 mg/ml, the percentage yield of particles at a constant Na alginate concentration increased by over 10 % and the corresponding encapsulation efficiency doubled. FTIR spectroscopic studies revealed the presence of folic acid within Na alginate matrices and also no characteristic chemical interaction between them. It can be concluded from the above research findings that, at 10 mg/ml Na alginate concentration, 10 mu l/min flow rate, and 12 kV voltage, a high amount of folic acid (5 mg/ml) can be encapsulated within Na alginate matrices, with high percentage yield (70 %) and loading capacity (96 %), generating non-spherical dried beads/particles of 90-150 nm in diameter.