Genome-scale screening and molecular characterization of membrane-bound transcription factors in Arabidopsis and rice
Genome-scale screening and molecular characterization of membrane-bound transcription factors in Arabidopsis and rice
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DOI：
10.1016/j.ygeno.2009.09.003
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发表时间：
2010-01-01
期刊：
影响因子：
4.4
通讯作者：
Park, Chung-Mo
中科院分区：
文献类型：
--
作者：
Kim, Sang-Gyu;Lee, Sangmin;Park, Chung-Mo
膜结合转录因子（MTF）的受控蛋白水解激活最近成为植物对环境变化快速转录反应的通用方法。在这里，我们报告了拟南芥和水稻基因组中假定的 MTF 的基因组规模鉴定。拟南芥和水稻基因组在几乎所有主要转录因子家族中分别至少有 85 个和 45 个 MTF。特别令人感兴趣的是 NAC MTF（指定为 NTL）：拟南芥中至少有 18 个 NTL，水稻中至少有 5 个 NTL 成员 (OsNTL)。虽然全尺寸 OsNTL 形式与膜相关，但缺乏跨膜结构域的截短形式仅在细胞核中检测到。此外，OsNTL 基因的转录水平在非生物胁迫处理后升高，支持它们在植物胁迫反应中的作用。我们认为膜介导的转录控制是基因调控网络的关键组成部分，可作为不利生长条件下的适应性策略。 (C) 2009 Elsevier Inc. 保留所有权利。
Controlled proteolytic activation of membrane-bound transcription factors (MTFs) is recently emerging as a versatile way of rapid transcriptional responses to environmental changes in plants. Here, we report genome-scale identification of putative MTFs in the Arabidopsis and rice genomes. The Arabidopsis and rice genomes have at least 85 and 45 MTFs, respectively, in virtually all major transcription factor families. Of particular interest is the NAC MTFs (designated NTLs): there are at least 18 NTLs in Arabidopsis and 5 NTL members (OsNTLs) in rice. While the full-size OsNTL forms are associated with the membranes, truncated forms lacking the transmembrane domains are detected exclusively in the nucleus. Furthermore, transcript levels of the OsNTL genes were elevated after treatments with abiotic stresses, supporting their roles in plant stress responses. We propose that membrane-mediated transcriptional control is a critical component of gene regulatory network that serves as an adaptive strategy under unfavorable growth conditions. (C) 2009 Elsevier Inc. All rights reserved.