Synergism between calcium-mediated and cyclic AMP-mediated activation of chloride secretion in isolated guinea pig distal colon.
Synergism between calcium-mediated and cyclic AMP-mediated activation of chloride secretion in isolated guinea pig distal colon.
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离体豚鼠远端结肠中钙介导和环 AMP 介导的氯离子分泌激活之间的协同作用。
DOI：
10.2170/jjphysiol.38.427
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Y. Suzuki
中科院分区：
文献类型：
--
作者：
T. Yajima;T. Suzuki;Y. Suzuki
用Ussing小室研究了钙离子和环状AMP介导的促分泌剂对豚鼠远端结肠氯离子分泌的协同作用。测量了22Na+和36Cl-的短路电流(ISC)和双向通量。前列腺素E_2(10(-7)-10(-6)M)、血管活性肠多肽(5×10(-9)-10(-8)M)或8-溴环腺苷(5×10(-4)-2×10(-3)M)可使ISC增加4-20倍。对~(36)Cl通量的测量表明，在8-溴环AMP和苯二酚的存在下，ISC的增加主要是由于Cl-分泌的增加。在此条件下，22Na的净吸收通量不受影响。A23187(5×10(-6)M)和8-溴环AMP(10(-3)M)联合使用也能增强ISC的增加。这一结果与同时激活环状AMP介导的系统和钙离子介导的系统可协同增加结肠的氯离子分泌的观点是一致的。
Synergistic effects of Ca2+-mediated secretagogues and cyclic AMP-mediated secretagogues on Cl- secretion by guinea pig distal colon were studied in vitro using Ussing chambers. Short-circuit current (Isc) and bidirectional fluxes of 22Na+ and 36Cl- were measured. Bethanechol (10(-4) M, a Ca2+-mediated secretagogue) caused an increase in Isc, which was enhanced 4-20 fold when the tissue was pretreated with prostaglandin E2 (10(-7)-10(-6) M), vasoactive intestinal polypeptide (5 x 10(-9)-10(-8) M) (cyclic AMP-mediated secretagogues), or 8-bromocyclic AMP (5 x 10(-4)-2 x 10(-3) M). Measurement of 36Cl flux showed that the increase in Isc in the presence of both 8-bromocyclic AMP and bethanechol resulted principally from increased Cl- secretion. On the other hand, the net absorptive flux of 22Na was not influenced under these conditions. Potentiation of the increase in Isc was also elicited by the combination of A23187 (5 x 10(-6) M) and 8-bromocyclic AMP (10(-3) M). The results are consistent with the notion that simultaneous activation of the cyclic AMP-mediated and Ca2+-mediated systems produces a synergistic increase in colonic Cl- secretion.
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A23187 诱导的结肠 K 和 Cl 转运变化是由不同的机制介导的。
DOI：
10.1152/ajpgi.1984.247.6.g695
发表时间：
1984
期刊：
The American journal of physiology
影响因子：
--
作者：
Smith,PL;McCabe,RD
通讯作者：
McCabe,RD
Ca2 控制主动肠道 Na 和 Cl 转运：参与神经体液作用。
DOI：
10.1152/ajpgi.1983.245.2.g165
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Donowitz,M
通讯作者：
Donowitz,M
人结肠上皮细胞系中的环 AMP 和 Ca2 激活 K 转运。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McRoberts,JA;Beuerlein,G;Dharmsathaphorn,K
通讯作者：
Dharmsathaphorn,K
刺激豚鼠远端结肠粘膜下神经元引起的上皮反应。
DOI：
10.1152/ajpgi.1987.252.5.g667
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Kuwahara,A;Bowen,S;Wang,J;Condon,C;Cooke,HJ
通讯作者：
Cooke,HJ