IMPROVED QUANTITATION AND DISCRIMINATION OF SULFATED GLYCOSAMINOGLYCANS BY USE OF DIMETHYLMETHYLENE BLUE
IMPROVED QUANTITATION AND DISCRIMINATION OF SULFATED GLYCOSAMINOGLYCANS BY USE OF DIMETHYLMETHYLENE BLUE
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DOI：
10.1016/0304-4165(86)90306-5
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发表时间：
1986-09-04
期刊：
影响因子：
--
通讯作者：
BARRETT, AJ
中科院分区：
文献类型：
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作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
硫酸化糖胺聚糖的二甲基亚甲基蓝测定法作为一种快速简便的测定组织和体液中硫酸化糖胺聚糖含量的方法已被广泛接受。然而，现有的测定方法在存在其他聚阴离子的情况下缺乏对硫酸化糖胺聚糖的特异性，并且不能区分不同的硫酸化糖胺聚糖。我们现在描述了一种改进的二甲基亚甲基蓝测定法，它对硫酸化糖胺聚糖具有更高的特异性，我们表明，与特定的多糖酶结合，二甲基亚甲基蓝测定法可用于定量单个硫酸化糖胺聚糖。
The dimethylmethylene blue assay for sulphated glycosaminoglycans has found wide acceptance as a quick and simple method of measuring the sulphated glycosaminoglycan content of tissues and fluids. The available assay methods have lacked specificity for sulphated glycosaminoglycans in the presence of other polyanions, however, and have not discriminated between the different sulphated glycosaminoglycans. We now describe a modified form of the dimethylmethylene blue assay that has improved specificity for sulphated glycosaminoglycans, and we show that in conjunction with specific polysaccharidases, the dimethylmethylene blue assay can be used to quantitate individual sulphated glycosaminoglycans.