Genes determining yeast replicative life span in a long-lived genetic background
Genes determining yeast replicative life span in a long-lived genetic background
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DOI：
10.1016/j.mad.2004.10.007
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发表时间：
2005-04-01
影响因子：
5.3
通讯作者：
Kennedy, BK
中科院分区：
文献类型：
--
作者：
Kaeberlein, M;Kirkland, KT;Kennedy, BK
在这里，我们描述了50多个同类酿酒酵母菌株的复制寿命，每个菌株携带一个突变，以前涉及酵母老化。这种分析提供了一个直接的比较，在一个单一的，长寿命的菌株背景下，大多数报道的酵母老化基因。在先前报道的增加酵母寿命的11种缺失突变中，我们发现FOB 1的缺失、SCH 9的缺失以及GPA 2、GPR 1或HXK 2的缺失（三种卡路里限制的遗传模型）显著增加了寿命。此外，SIR2的过度表达或低葡萄糖生长延长了寿命。这些结果定义了有限数量的基因可能以不依赖于菌株的方式调节复制寿命，并为未来的上位性分析奠定了基础，以确定衰老的遗传途径。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
Here we describe the replicative life spans of more than 50 congenic Saccharomyces cerevisiae strains, each carrying a mutation previously implicated in yeast aging. This analysis provides a direct comparison, in a single, long-lived strain background, of a majority of reported yeast aging genes. Of the eleven deletion mutations previously reported to increase yeast life span, we find that deletion of FOB1, deletion of SCH9, and deletion of GPA2, GPR1, or HXK2 (three genetic models of calorie restriction) significantly enhanced longevity. In addition, overexpression of SIR2 or growth on low glucose increased life span. These results define a limited number of genes likely to regulate replicative life span in a strain-independent manner, and create a basis for future epistasis analysis to determine genetic pathways of aging. (c) 2004 Elsevier Ireland Ltd. All rights reserved.