Cutting edge:: Primary B lmphocytes preferentially expand allogeneic FoxP3+ CD4 T cells
Cutting edge:: Primary B lmphocytes preferentially expand allogeneic FoxP3+ CD4 T cells
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DOI：
10.4049/jimmunol.179.4.2046
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发表时间：
2007-08-15
影响因子：
4.4
通讯作者：
Jensen, Peter E.
中科院分区：
文献类型：
--
作者：
Chen, Xinjian;Jensen, Peter E.
尽管B淋巴细胞在体液免疫中作为效应细胞的作用是明确的，但已有研究报道B细胞具有耐受性。B细胞介导的耐受的影响及其潜在机制尚不完全清楚。用原代B细胞作为APC，同种异体CD4T细胞作为混合白细胞反应的反应细胞，我们发现在没有外源性细胞因子的情况下，B细胞优先扩增ToxP3(+)而不是FoxP3(-)的CD4T细胞。部分阻断H类MHC-TCR相互作用可进一步增强Foxp3(+)T细胞的优先扩增，但可被刺激的抗CD28抗体或在高B/T细胞比率时减弱。外源性IL-2可选择性地抑制B细胞扩增CD25(+)而非CD25(-)CD4T细胞的能力。B细胞扩增的CD25+T细胞表达高水平的FoxP3，并以抗原特异的方式高度抑制。
Despite the unequivocal role of B lymphocytes as effecter cells in humoral immunity, studies have reported that B cells are tolerogenic. The impact of B cell-mediated tolerance and its underlying mechanisms are incompletely understood. Using primary B cells as APCs and allogeneic CD4 T cells as responder cells in mixed leukocyte reactions, we find that B cells preferentially expand ToxP3(+) over FoxP3(-) CD4 T cells in the absence of exogenous cytokines. The preferential expansion of Foxp3(+) T cells is further enhanced by a partial blockade of class H MHC- TCR interaction but diminished by stimulatory anti-CD28 Ab or at high B to T cell ratios. Gamma irradiation of B cells selectively abrogates their ability to expand isolated CD25(+) but not CD25(-) CD4 T cells, exogenous IL-2 supplement can partially restore this function. B cell-expanded CD25+ T cells express high levels of FoxP3 and are highly inbibitoryin an Ag-specific manner.