Analysis of Global Expression Profiles of Arabidopsis Genes Under Abscisic Acid and H2O2 Applications
Analysis of Global Expression Profiles of Arabidopsis Genes Under Abscisic Acid and H2O2 Applications
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DOI：
10.1111/j.1744-7909.2006.00213.x
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发表时间：
2006
影响因子：
11.4
通讯作者：
Pengcheng Wang;Yanyan Du;G. An;Yun Zhou;Chen Miao;Chunpeng Song
中科院分区：
文献类型：
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作者：
Pengcheng Wang;Yanyan Du;G. An;Yun Zhou;Chen Miao;Chunpeng Song
为了深入了解脱落酸（阿坝）和H2 O2应用下基因表达谱的协调，使用GeneChip（Santa Clara，CA，USA）阵列研究了拟南芥幼苗中响应阿坝和H2 O2的基因表达的全局变化。在24 000多个基因中，459个转录本在H2 O2处理后显著增加，而另外221个转录本在H2 O2处理后显著减少。与H_2O_2处理相似，阿坝处理提高了391个基因的转录，抑制了322个基因的转录。两种处理共有143个上调基因和75个下调基因，这些基因主要参与代谢、信号转导、转录、防御和抗性等过程。只有两个基因，编码APETALA 2/脱水反应元件结合蛋白（AP 2/DREBP）家族转录因子和晚期胚胎发育丰富的蛋白质，被H2 O2下调，但上调阿坝。这些结果表明，与阿坝类似，H2 O2在拟南芥幼苗的基因转录中起着全局性作用。外源阿坝和H_2O_2诱导的转录反应基本重叠。这两种处理以协调的方式调节大多数下游基因。（责任编辑：赵丽慧）
To gain insight into the coordination of gene expression profiles under abscisic acid (ABA) and H2O2 applications, global changes in gene expression in response to ABA and H2O2 in Arabidopsis seedlings were investigated using GeneChip (Santa Clara, CA, USA) arrays. Among over 24 000 genes present in the arrays, 459 transcripts were found to be significantly increased, whereas another 221 decreased following H2O2 treatment compared with control. Similar to treatment with H2O2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydration-responsive element binding protein (AP2/DREBP) family transcriptional factor and a late embryogenesis-abundant protein, were downregulated by H2O2, but upregulated by ABA. These results suggest that, similar to ABA, H2O2 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H2O2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner. (Managing editor: Li-Hui Zhao)