Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
复制标题
灰霉鬼伞糖苷水解酶家族 6 多基因的克隆和转录本分析
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
K. Fukuda
中科院分区：
文献类型：
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作者：
Makoto Yoshida;Kan Sato;S. Kaneko;K. Fukuda
我们检索了担子菌灰盖鬼伞的基因组数据库（Coprinus cinereus）中，发现了编码糖苷水解酶家族6（GH 6）酶的五个基因，CcCel 6A、CcCel 6 B、CcCel 6C、CcCel 6D和CcCel 6 E，按基因座数目递增的顺序命名（CC1G_01107.1、CC1G_04166.1、CC1G_08276.1、CC1G_08277.1和CC1G_10605.1）。CcCel 6A的氨基酸序列表明由N-末端家族1碳水化合物结合模块（CBM 1）和GH 6催化结构域组成的双结构域结构，而其他基因缺乏CBM 1。CcCel 6A的转录本在纤维素培养的活跃生长阶段被观察到，而它们在葡萄糖培养中不存在。纤维二糖强烈诱导CcCel 6A的转录。另一方面，在葡萄糖和纤维素培养物中检测到CcCel 6 B、-D和-E的转录物，并且它们的转录被纤维二糖弱诱导。CcCel 6C的转录水平不受葡萄糖或纤维二糖的影响。
We searched the genome database of the basidiomycete Coprinopsis cinerea (Coprinus cinereus) and found five genes encoding the glycoside hydrolase family 6 (GH6) enzyme, CcCel6A, CcCel6B, CcCel6C, CcCel6D, and CcCel6E, designated in order of increasing locus number (CC1G_01107.1, CC1G_04166.1, CC1G_08276.1, CC1G_08277.1, and CC1G_10605.1). The amino acid sequence of CcCel6A suggests a two-domain structure consisting of an N-terminal family 1 carbohydrate-binding module (CBM1) and a GH6 catalytic domain, while the other genes lack CBM1. The transcripts of CcCel6A were observed at the active growth stage in cellulose culture, whereas they were absent from glucose culture. Cellobiose strongly induced transcription of CcCel6A. On the other hand, transcripts of CcCel6B, -D, and -E were detected in both glucose and cellulose cultures, and transcription of them was induced weakly by cellobiose. The transcript level of CcCel6C was not influenced by glucose or cellobiose.
影响因子：
2.9
作者：
Barr, BK;Hsieh, YL;Wilson, DB
通讯作者：
Wilson, DB