Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines
Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines
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DOI：
10.1016/j.vetimm.2004.11.003
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发表时间：
2005-03-10
影响因子：
1.8
通讯作者：
Smith, AL
中科院分区：
文献类型：
--
作者：
Iqbal, M;Philbin, VJ;Smith, AL
细胞表面分子的Toll样受体（TLR）家族代表模式识别系统的主要组成部分，其使脊椎动物和无脊椎动物能够检测入侵的微生物并产生抗微生物反应。小鼠和人的TLR库已被深入研究，在本文中，我们报告了与chTLR 5和chTLR 7同源的EST的鉴定，并独立确认了EST数据库中chTLR 1/6/10和3的鉴定。我们已经确定了7个鸡类TLR（chTLR）在广泛的鸡组织，分离的免疫细胞类型和培养细胞的mRNA表达模式。一些chTLR在大多数组织中表达（chTLR 1/6/10、chTLR 3、chTLR 4和chTLR 5），而另一些chTLR表现出更受限的表达模式（chTLR 2 1型、2型和chTLR 7）。从外周血或脾分离的先天性和适应性免疫细胞类型以及体细胞或免疫来源的培养细胞中观察到类似的不同chTLR表达模式。对不同组织、免疫细胞亚群和培养细胞类型的TLR库的理解允许对响应鸡病原体的免疫诱导进行更精细的解释。(C)2004 Elsevier B. V.保留所有权利。
The Toll-like receptor (TLR) family of cell surface molecules represent a major component of the pattern recognition system, which enables both vertebrates and invertebrates to detect invading microorganisms and mount an anti-microbial response. The TLR repertoire of mouse and man has been intensively studied and in this manuscript we report the identification of ESTs with homology to chTLR5 and chTLR7, and independently confirm the identification of chTLR 1/6/10 and 3 in the EST databases. We have determined the mRNA expression patterns for seven chicken TLRs (chTLR) in a wide range of chicken tissues, isolated immune cell types and cultured cells. Some of the chTLR were expressed in most tissues (chTLR1/6/10, chTLR3, chTLR4 and chTLR5), whereas others exhibited more restricted expression patterns (chTLR2 type 1, type 2 and chTLR7). Similarly distinct patterns of chTLR expression were seen with innate and adaptive immune cell types isolated from peripheral blood or spleen and with cultured cells of somatic or immunological origin. An understanding of the TLR repertoire for different tissues, immune cell subsets and cultured cell types allows more refined interpretation of immune induction in response to chicken pathogens. (C) 2004 Elsevier B.V. All rights reserved.