Whole cell bioreporter application for rapid detection and evaluation of crude oil spill in seawater caused by Dalian oil tank explosion.
Whole cell bioreporter application for rapid detection and evaluation of crude oil spill in seawater caused by Dalian oil tank explosion.
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DOI：
10.1016/j.watres.2012.11.038
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发表时间：
2013-03
期刊：
影响因子：
12.8
通讯作者：
Dayi Zhang;A. Ding;Shuangchao Cui;Cheng Hu;S. Thornton;Junfeng Dou;Yujiao Sun;Wei E. Huang
中科院分区：
文献类型：
--
作者：
Dayi Zhang;A. Ding;Shuangchao Cui;Cheng Hu;S. Thornton;Junfeng Dou;Yujiao Sun;Wei E. Huang
原油泄漏到海水中的事故对人类和动物健康、渔业和海洋生态系统构成严重威胁。全细胞生物报告基因检测方法具有快速评价原油污染场所毒性和生物利用度的独特优势，是开展原油污染场所环境风险评价的有效工具。贝氏不动杆菌ADPWH_alk和ADPWH_recA是基于染色体的烷烃和遗传毒性生物报告基因，在烷烃和遗传毒性化合物存在下可被激活表达生物发光。在这项研究中，我们应用不动杆菌ADPWH_alk和ADPWH_recA生物报告菌对2010年中国大连油罐爆炸后四周的大连湾周围的6个海水和6个沉积物样本进行了检测，并将结果与一年后同一地点的样本进行了比较。生物报告基因检测结果表明，泄漏后4周，DB、NT、JSB、XHP和FJZ 5个站点的海水和沉积物受到原油污染，具有不同程度的遗传毒性。在这6个地点中，DB和NT具有高含油量和高遗传毒性，而JSB与位于半岛另一侧的未污染地点LSF相比，含油量高但遗传毒性低。这三个可检测到遗传毒性的地点（DB、NT和JSB）距离溢油点30公里以内。距离较远的XHP （38.1 km）和FJZ （31.1 km）两个站点被石油轻微污染，但没有遗传毒性，表明它们处于污染边界附近。生物报告检测还表明，所有六个地点在漏油一年后都是干净的，因为烷烃和遗传毒性低于检测限值。该研究表明，生物报告基因检测可作为原油泄漏事故规模估算、污染海水和沉积物生物利用度和遗传毒性评估的快速方法，对环境管理和清理的风险评估和战略决策具有重要意义。
Accidents involving the release of crude oil to seawater pose serious threat to human and animal health, fisheries and marine ecosystems. A whole cell bioreporter detection method, which has unique advantages for the rapid evaluation on toxicity and bioavailability, is a useful tool to provide environmental risk assessments at crude oil-contaminated sites. Acinetobacter baylyi ADPWH_alk and ADPWH_recA are chromosomally-based alkane and genotoxicity bioreporters which can be activated to express bioluminescence in the presence of alkanes and genotoxic compounds. In this study, we applied Acinetobacter ADPWH_alk and ADPWH_recA bioreporters to examine six seawater and six sediment samples around the Dalian Bay four weeks after an oil tank explosion in Dalian, China in 2010, and compared the results with samples from the same sites one year after. The results of bioreporter detection suggest that seawater and sediments from five sites (DB, NT, JSB, XHP and FJZ) four weeks after the oil-spill were contaminated by the crude oil with various extents of genotoxicity. Among these six sites, DB and NT had high oil contents and genotoxicity, and JSB had high oil content but low genotoxicity in comparison with an uncontaminated site LSF, which is located at other side of the peninsula. These three sites (DB, NT and JSB) with detectable genotoxicity are within 30 km away from the oil spill point. The far-away two sites XHP (38.1 km) and FJZ (31.1 km) were lightly contaminated with oil but no genotoxicity suggesting that they are around the contamination boundary. Bioreporter detection also indicates that all six sites were clean one year after the oil-spill as the alkane and genotoxicity were below detection limit. This study demonstrates that bioreporter detection can be used as a rapid method to estimate the scale of a crude oil spill accident and to evaluate bioavailability and genotoxicity of contaminated seawater and sediments, which are crucial to risk assessment and strategic decision-making for environmental management and clean-up.