Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway
Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway
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DOI：
10.1111/j.1574-695x.2010.00731.x
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发表时间：
2010-11-01
影响因子：
--
通讯作者：
Azuma, Takeshi
中科院分区：
文献类型：
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作者：
Nobutani, Kentaro;Yoshida, Masaru;Azuma, Takeshi
海尔曼螺杆菌诱导小鼠胃淋巴滤泡。然而，H.尚未阐明Heilmannii感染。本研究旨在探讨派尔集合淋巴结（PP）在H。Heilmannii诱导的免疫应答和胃淋巴滤泡的发育。用H. Heilmannii，并且除了组织学和免疫组织学检查之外，还研究了胃粘膜中细胞因子和趋化因子的表达水平。感染后1个月，PP缺陷小鼠胃淋巴滤泡形成和树突状细胞、B细胞和辅助性T细胞浸润较轻，但3个月后两种类型小鼠的淋巴滤泡形成和树突状细胞、B细胞和辅助性T细胞浸润相似。肿瘤坏死因子α和CC趋化因子配体2的mRNA表达水平在H.感染后1个月，感染的C57 BL/6 J野生型小鼠中CXC趋化因子配体13的表达显著增加。提示PP对H诱导胃淋巴滤泡的形成和发育不是必需的。Heilmannii感染，尽管它们参与胃淋巴滤泡形成的速度。
Helicobacter heilmannii induces gastric lymphoid follicles in mice. However, the pathogenic mechanisms behind the induction of gastric lymphoid follicles by H. heilmannii infection have not been elucidated. The aim of this study was to investigate the roles of Peyer's patches (PP) in H. heilmannii-induced immune responses and the development of gastric lymphoid follicles. C57BL/6J and PP deficient mice were infected with H. heilmannii, and in addition to histological and immunohistological examinations, the expression levels of cytokines and chemokines in gastric mucosa were investigated. Gastric lymphoid follicle formation and the infiltration of dendritic cells, B cells, and helper T cells were milder in the PP-deficient mice 1 month after infection, but they were similar in both types of mice after 3 months. The mRNA expression levels of tumor necrosis factor alpha and CC chemokine ligand 2 were significantly high in the H. heilmannii-infected groups, and CXC chemokine ligand 13 expression was significantly increased in the infected C57BL/6J wild-type mice 1 month after infection. These results suggest that PP are not essential for the formation and development of gastric lymphoid follicles induced by H. heilmannii infection, although they are involved in the speed of gastric lymphoid follicle formation.