A ruthenium(II) polypyridyl complex for direct imaging of DNA structure in living cells
A ruthenium(II) polypyridyl complex for direct imaging of DNA structure in living cells
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DOI：
10.1038/nchem.406
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发表时间：
2009-11-01
期刊：
影响因子：
21.8
通讯作者：
Thomas, Jim A.
中科院分区：
文献类型：
--
作者：
Gill, Martin R.;Garcia-Lara, Jorge;Thomas, Jim A.
在寻找新的生物成像剂中，能够从三重态金属-配体电荷转移（MLCT）态发光的金属配合物作为DNA结构的发光探针提供了许多优点。然而，差的细胞摄取限制了它们在活细胞中的使用。在这里，我们提出了一个双核钌（II）多吡啶系统，作为一个多功能的生物成像剂染色的真核和原核细胞的DNA发光和透射电子显微镜。这种MLCT“光开关”复合物直接成像活细胞的核DNA，而不需要预先进行膜透化。此外，抑制和透射电子显微镜研究表明，这是通过一个非内吞，但温度依赖性，在MCF-7细胞中的细胞摄取机制，和共聚焦显微镜揭示了多个发射峰，作为细胞DNA结构的标记。
In the search for new biological imaging agents, metal coordination compounds able to emit from triplet metal-to-ligand charge transfer (MLCT) states offer many advantages as luminescent probes of DNA structure. However, poor cellular uptake restricts their use in live cells. Here, we present a dinuclear ruthenium(II) polypyridyl system that works as a multifunctional biological imaging agent staining the DNA of eukaryotic and prokaryotic cells for both luminescence and transition electron microscopy. This MLCT 'light switch' complex directly images nuclear DNA of living cells without requiring prior membrane permeabilization. Furthermore, inhibition and transmission electron microscopy studies show this to be via a non-endocytotic, but temperature-dependent, mechanism of cellular uptake in MCF-7 cells, and confocal microscopy reveals multiple emission peaks that function as markers for cellular DNA structure.