High-performance liquid chromatographic fingerprint analysis for different origins of sea buckthorn berries
High-performance liquid chromatographic fingerprint analysis for different origins of sea buckthorn berries
复制标题
DOI：
10.1016/j.chroma.2007.03.097
复制
发表时间：
2007-06-22
影响因子：
4.1
通讯作者：
Bai, Nan
中科院分区：
文献类型：
--
作者：
Chen, Chu;Zhang, Hao;Bai, Nan
采用高效液相色谱法建立了沙棘果实黄酮类化合物的化学指纹图谱，并对不同产地沙棘果实黄酮类化合物的差异进行了研究。对34个样品进行了分析，其中包括15个RS（Hippophae rhamnoides ssp. sinensis）样品，7个RY（H.鼠李属yunnanensis）样品，5个RW（H.拟鼠李亚种wolongensis）样品，4个NS（H.脉果属stellatopilosa）样品和3个TI（H.西藏）样品。从槲皮素中分离得到12个黄酮类化合物，分别为槲皮素3-O-槐糖苷-7-鼠李糖苷、山奈酚3-O-槐糖苷-7-O-鼠李糖苷、异鼠李素3-O-槐糖苷-7-O-鼠李糖苷、异鼠李素3-O-葡萄糖苷-7-O-鼠李糖苷、槲皮素3-O-芸香糖苷、槲皮素3-O-葡萄糖苷、异鼠李素3-O-芸香糖苷、异鼠李素3-O-葡萄糖苷、槲皮素、山奈酚7-O-鼠李糖苷、山奈酚和异鼠李素。计算色谱相似度相关系数和特征化合物的相对峰面积，定量表达HPLC指纹图谱。结果表明，相似度评价结合指纹图谱可以有效地鉴别和区分不同品种的沙棘果。但RS和RY之间的差异不明显，表明这两个亚种在化学分类学上可能有很近的关系。该方法适用于沙棘果及其提取物的指纹图谱分析，可用于沙棘果及其提取物的真伪鉴别和质量控制。(c)2007 Elsevier B. V.保留所有权利。
Using high-performance liquid chromatography (HPLC), a chemical fingerprint method was developed for investigating and demonstrating the variance of flavonoids among different origins of sea buckthorn berries. Thirty-four samples were analyzed including 15 RS (Hippophae rhamnoides ssp. sinensis) samples, 7 RY (H. rhamnoindes ssp. yunnanensis) samples, 5 RW (H. rhamnoides ssp. wolongensis) samples, 4 NS (H. neurocarpa ssp. stellatopilosa) samples and 3 TI (H. tibetana) samples. In the HPLC chromatograms, 12 compounds were identified as flavonoids, including quercetin 3-O-sophoroside-7-rhamnoside, kaempferol 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-sophoroside7-O-rhamnoside, isorhamnetin 3-O-glucoside-7-O-rhamnoside, quercetin 3-O-rutinoside, quercetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, isorhamnetin 3-O-glucoside, quercetin, kaempferol 7-O-rhamnoside, kaempferol and isorhamnetin. Both correlation coefficient of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish sea buckthorn berries from different species. However, no obvious difference between RS and RY suggested that the two subspecies might have very close relationship in terms of chemotaxonomy. The established method was considered to be suitable for fingerprint analysis to check the genuine origin and control the quality of sea buckthorn berries and extracts. (c) 2007 Elsevier B.V. All rights reserved.