Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis.
Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis.
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对人类免疫缺陷病毒感染患者外周血细胞中的人类巨细胞病毒 DNA 进行定量可以预测巨细胞病毒性视网膜炎。
DOI：
10.1093/infdis/171.1.177
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Merigan,TC
中科院分区：
文献类型：
--
作者：
Rasmussen,L;Morris,S;Zipeto,D;Fessel,J;Wolitz,R;Dowling,A;Merigan,TC
Human cytomegalovirus (CMV) DNA copy number in white blood cells from both human immunodeficiency virus (HIV)-seronegative and HIV-seropositive patients was amplified from the immediate-early region of CMV DNA and quantified by colorimetric detection of the hybridization of the amplification product to a detector oligonucleotide probe in microtiter wells. By Mann-WhitneyUtest, significantly higher (P< .05, two-tailed) copy numbers of CMV DNA were detected in HIV-seropositive patients with retinitis than in either patients with < 100 CD4 cells/mm3and no symptomatic CMV disease or HIV-seropositive patients with > 100 CD4 cells/mm3. By prospective monitoring for increases in CMV DNA copy number, it may be possible to identify HIV-seropositive patients who are at imminent risk for development of symptomatic CMV retinitis.
