An electrochemical immunoassay for protein based on bio bar code method
An electrochemical immunoassay for protein based on bio bar code method
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基于生物条形码法的蛋白质电化学免疫分析
DOI：
10.1016/j.bios.2008.12.023
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发表时间：
2009-04-15
影响因子：
12.6
通讯作者：
Zhang, Shusheng
中科院分区：
文献类型：
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作者：
Ding, Caifeng;Zhang, Qian;Zhang, Shusheng
基于DNA修饰金纳米粒子（Au NPs）的电化学生物条形码分析提供了一种非酶的蛋白质定量检测方法。通过将抗原（Ag）夹在抗体（Ab）修饰的磁珠（MB）和与第二Ab和生物条形码DNA双功能的放大器Au NP之间，建立了检测方法。随后的电化学溶出分析从CdS NPs上溶解的镉提供了一种量化分析物中银浓度的方法。使用AFP检测作为模型，我们证明了在0.02至3.5 ng/mL范围内的线性剂量响应。该方法测定AFP的检出限为9.6 pg/mL，较最佳酶联免疫吸附试验（ELISA）系统提高100倍。此外，还首次测定了每个MB上加载的抗afp的数量，以及每个Au NP上修饰的二级抗afp和生物条形码DNA的数量。MB和anti-AFP的比值约为1/ 16700，Au NPs、anti-AFP、条形码DNA探针和CdS NPs的比值约为1/16/101/77。该方法具有较高的灵敏度和选择性，可用于临床免疫分析。(C) 2008 Elsevier B.V.版权所有
An electrochemical bio bar code assay based on DNA modified gold nanoparticles (Au NPs) provides a nonenzymatic method for quantitative detection of protein. By sandwiching an antigen (Ag) between a magnetic bead (MB) modified with antibody (Ab) and an amplifier Au NP bifunctioned with second Ab and bio bar code DNA, the detection method was established. Subsequent electrochemical stripping analysis of the cadmium dissolved from CdS NPs on the congregates provided a means to quantify the concentration of Ag in the analyte. Using AFP detection as a model, we demonstrate a linear dose response in the range from 0.02 to 3.5 ng/mL. The detection limit of this assay for AFP determined is 9.6 pg/mL, 100-fold improvement compared to the best enzyme-linked immunosorbent assay (ELISA) system. in addition, the amount of anti-AFP loaded on one MB, and the numbers of the secondary anti-AFP and bio bar code DNA modified on one Au NP were all determined for the first time. The ratio of MB and anti-AFP was approximately 1/16,700, and the ratios of Au NPs, anti-AFP, bar code DNA probes, and CdS NPs were about 1/16/101/77. It would be valuable for clinical immunoassay due to the high sensitivity and selectivity. (C) 2008 Elsevier B.V. All rights reserved.