Malvastrum yellow vein virus,a new Begomovirus species associated with satellite DNA molecule
Malvastrum yellow vein virus,a new Begomovirus species associated with satellite DNA molecule
复制标题
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
2.5
通讯作者：
ZHOUXuepinq;XieYan;Peng-yan;ZHANGZhongkai
中科院分区：
文献类型：
--
作者：
ZHOUXuepinq;XieYan;Peng-yan;ZHANGZhongkai
从云南省红河县的赛葵上分离到病毒分离物Y 47。DNA-A基因全长2731个核苷酸，具有典型的菜豆花叶病毒基因组结构，编码6个开放阅读框，其中2个为病毒体正义DNA的开放阅读框[AV 1（CP）和AV 2]，4个为互补正义DNA的开放阅读框（AC 1-AC 4）。比较结果表明，Y 47与黄秋葵黄脉花叶病毒-[201]（AJ 002451）的总DNA-A序列同源性最高（77%），而与其他菜豆花叶病毒的同源性低于76%。分子数据表明，病毒分离物Y 47是一个独特的菜豆病毒种，因此建议将其命名为赛葵黄脉病毒。利用DNAβ特异性引物（beta 01和beta 02）发现卫星DNA分子（Y 47 β）与Y 47相关。Y 47 β基因全长1348个核苷酸，在互补链DNA中有一个功能性的ORF（C1）。Y 47 β与棉花曲叶木尔坦病毒和棉花曲叶拉贾斯坦邦病毒的DNAβ分子的同源性为62%~ 67%，而与其它已报道的DNAβ分子的同源性低于46%。亲缘关系树状图显示，DNAβ分子与菜豆花叶病毒属病毒是协同进化的。
Virus isolate Y47 was obtained from Malvastrum coromandelianum showing yellow vein symptom in Honghe, Yunnan Province. The complete nucleotide sequence of DNA-A was determined, it contains 2731 nucleotides, having typical genomic organization of a begomovirus, encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion- sense DNA and 4 ORFs (AC1-AC4) in complementary- sense DNA. Comparisons show that the total DNA-A of Y47 has the highest sequence identity (77%) with that of Okra yellow vein mosaic virus-[201] (AJ002451), while less than 76% identities are found when compared with other begomoviruses. The molecular data show that virus isolate Y47 is a distinct begomovirus species, for which the name Malvastrum yellow vein virus is proposed. Satellite DNA molecule (Y47β) was found to be associated with Y47 using the primers (beta01 and beta02) specific for DNAβ. Y47β consists of 1348 nucleotides, with a functional ORF (C1) in complementary-sense DNA. Y47β Has 62%-67% sequence identity with DNAβ molecule associated with Cotton leaf curl Multan virus or Cotton leaf curl Rajasthan virus, while lower than 46% sequence identities are found when compared with other reported DNAβ molecules. Relationship dendrograms show that DNAβ molecules are co-evolved with their help begomoviruses.