Ionic matrices for matrix-assisted laser desorption/ionization time-of-flight detection of DNA oligomers
Ionic matrices for matrix-assisted laser desorption/ionization time-of-flight detection of DNA oligomers
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DOI：
10.1002/rcm.931
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Armstrong, DW
中科院分区：
文献类型：
--
作者：
Carda-Broch, S;Berthod, A;Armstrong, DW
具有低熔点的盐，也称为室温离子液体，可以用作基质辅助激光解吸/电离飞行时间（MALDI-TOF）的基质。它们具有很强的真空稳定性，可以溶解极性和极性溶质，包括碳水化合物、生物低聚物和蛋白质。与固体基质相比，离子液体提供的样品溶液更加均匀。我们证明了使用离子基质通过直接TOF质谱分析来确定DNA低聚物分子量的有效性。用不同碱基合成的离子基质与两种酸（3-羟基苯甲酸和2,5-二羟基苯甲酸）结合，对三种寡核苷酸(d(pT)（10）、d(pC)（11）和d（pC)(12)）进行了测试。结果表明，在本实验条件下，最佳离子基质相对于常规分子基质能增强寡核苷酸的离子峰强度。在一种情况下，离子基质提供的信噪比比相应的分子基质高十倍。几个被测试的离子基质是液体。然而，所有工作的离子基质都是固体。版权所有：John Wiley Sons， Ltd。
Salts with low melting points, also termed room-temperature ionic liquids, can be used as matrices in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). They have great vacuum stability, and can dissolve polar and apolar solutes including carbohydrates, biological oligomers and proteins. The ionic liquids give much more homogeneous sample solutions compared with solid matrices. We demonstrate the usefulness of using ionic matrices to determine the molecular weight of DNA oligomers by direct TOF mass spectrometric analysis. Three oligonucleotides were tested, (d(pT)(10), d(pC)(11) and d(PC)(12)), with several ionic matrices synthesized from different bases associated to two acids (3-hydroxypicolinic acid and 2,5-dihydroxybenzoic acid). The results obtained show that the best ionic matrices enhance the ion peak intensity of the oligonucleotides with respect to conventional molecular matrices under our experimental conditions. In one case, an ionic matrix provided a signal-to-noise ratio ten times higher than the corresponding molecular matrix. Several of the tested ionic matrices were liquids. However, all working ionic matrices were solids. Copyright (C) 2003 John Wiley Sons, Ltd.