A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations
A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations
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DOI：
10.1074/jbc.m608228200
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发表时间：
2006-11-24
影响因子：
4.8
通讯作者：
Mueller, Matthias
中科院分区：
文献类型：
--
作者：
Nishiyama, Ken-ichi;Ikegami, Ayao;Mueller, Matthias
开发了一种无细胞系统，该系统允许将大肠杆菌的单跨膜蛋白和多跨膜蛋白正确整合到蛋白脂质体中。我们发现，需要生理水平的甘油二酯，以防止自发整合到脂质体中，即使是多面体甘露醇渗透酶。使用含有二酰基甘油的脂蛋白体，我们确定了一种新的整合刺激因子。甘露醇通透酶的整合依赖于SecYEG转座子和该因子，并由信号识别颗粒和信号识别颗粒受体介导。该因子也促进了M13前包衣的整合，其独立于信号识别颗粒/信号识别颗粒受体和SecYEG。此外，该因子刺激前分泌蛋白的翻译后易位，表明它也介导信号序列的整合。发现该因子是具有肽部分的脂质A衍生的膜组分。
A cell-free system was developed that allows the correct integration of single and multispanning membrane proteins of Escherichia coli into proteoliposomes. We found that physiological levels of diacylglycerol were required to prevent spontaneous integration into liposomes even of the polytopic mannitol permease. Using diacylglycerol-containing proteoliposomes, we identified a novel integration-stimulating factor. Integration of mannitol permease was dependent on both the SecYEG translocon and this factor and was mediated by signal recognition particle and signal recognition particle receptor. Integration of M13 procoat, which is independent of both signal recognition particle/signal recognition particle receptor and SecYEG, was also promoted by this factor. Furthermore, the factor stimulated the post-translational translocation of presecretory proteins, suggesting that it also mediates integration of a signal sequence. This factor was found to be a lipid A-derived membrane component possessing a peptide moiety.