STIMULATORY EFFECTS OF BRADYKININ ON THE OVULATORY PROCESS IN THE INVITRO-PERFUSED RAT OVARY
STIMULATORY EFFECTS OF BRADYKININ ON THE OVULATORY PROCESS IN THE INVITRO-PERFUSED RAT OVARY
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DOI：
10.1095/biolreprod44.2.269
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发表时间：
1991-02-01
影响因子：
3.6
通讯作者：
BRANNSTROM, M
中科院分区：
文献类型：
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作者：
HELLBERG, P;LARSON, L;BRANNSTROM, M
采用体外灌注大鼠卵巢模型研究缓激肽在排卵过程中的作用。用LH（0.1 μ g/ml）刺激导致每个卵巢排卵2.6 +/- 0.5（平均值+/- SEM），而未刺激的对照组没有排卵。缓激肽（5 μ M）加入到灌注系统每小时10小时诱导2的5个卵巢排卵，分别与2和3排卵。当在LH后5或10小时给予缓激肽（5 μ M）作为单次剂量时，排卵率显著增加，分别为每个卵巢11.0 +/- 2.8和8.6 +/- 2.0个排卵。一种竞争性缓激肽拮抗剂苯丙氨酸缓激肽可抑制缓激肽诱导的LH刺激排卵的增加，但不包括缓激肽，加入LH可增加颗粒细胞中前列腺素内过氧化物合酶的水平，但残留卵巢组织中的酶水平可忽略不计。相反，前列环素合成酶主要位于残留的卵巢组织中。这种酶不受LH或缓激肽的影响。LH在7 h时增加了前列腺素（主要是前列腺素E2（PGE 2））的组织水平，而缓激肽的刺激作用较小，前列环素（前列腺素I2）水平优先增加。这项研究表明，在大鼠中，缓激肽的调节作用，可能涉及排卵过程中的前列环素。
The role of bradykinin in the ovulatory process was investigated using an in vitro-perfused rat ovary model. Stimulation with LH (0.1-mu-g/ml) resulted in 2.6 +/- 0.5 (mean +/- SEM) ovulations per ovary, whereas no ovulations occurred in the nonstimulated control group. Bradykinin (5-mu-M) added to the perfusion system hourly for 10 h induced 2 of 5 ovaries to ovulate, with 2 and 3 ovulations, respectively. When bradykinin (5-mu-M) was given as a single dose at 5 or 10 h after LH, the ovulation rate was significantly increased to 11.0 +/- 2.8 and 8.6 +/- 2.0 ovulations per ovary, respectively. A competitive bradykinin antagonist, phenylalanine bradykinin, inhibited the bradykinin-induced increase in LH-stimulated ovulations.The addition of LH, but not of bradykinin, increased the levels of prostaglandin endoperoxide synthase in granulosa cells, but the levels of the enzyme in the residual ovarian tissue were negligible. In contrast, prostacyclin synthase was predominantly located in the residual ovarian tissue. This enzyme was not affected by LH or bradykinin.LH increased the tissue levels of prostaglandins, predominantly prostaglandin E2 (PGE2), at 7 h, whereas the stimulatory effect of bradykinin was smaller, with a preferential increase in prostacyclin (prostaglandin I2) levels. This study indicates a modulatory role of bradykinin, possibly involving prostacyclin late in the ovulatory process, in the rat.