The maturity index as a tool to facilitate the interpretation of changes in vitellogenin production and sex ratio in the Fish Sexual Development Test
The maturity index as a tool to facilitate the interpretation of changes in vitellogenin production and sex ratio in the Fish Sexual Development Test
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DOI：
10.1016/j.aquatox.2012.11.016
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发表时间：
2013-03-15
影响因子：
4.5
通讯作者：
Braunbeck, Thomas
中科院分区：
文献类型：
--
作者：
Baumann, Lisa;Holbech, Henrik;Braunbeck, Thomas
2011年7月，鱼类性发育测试（FSDT）正式被采纳为经合组织测试指南234，用于检测内分泌干扰物（EDCs）。性别比例和卵黄蛋白原（VTG）诱导是该测试中强制性的内分泌终点，而性腺分期仅作为一个选项。本研究用不同作用方式的EDCs （17 α -炔雌醇、二氢睾酮、17 β -trenbolone、prochloraz和4-叔戊基酚）对斑马鱼（Danio rerio）进行了5次FSDTs。结果表明，受污染的鱼类不仅性别比和VTG产量受到严重影响，性腺成熟度也受到严重影响。成熟度指数作为一种量化斑马鱼性腺成熟的新方法，不仅可以更好地评估edc对性腺成熟的剂量依赖性影响，还可以对组织学数据进行统计分析。VTG诱导与成熟度指数在5种EDCs中均表现出极好的相关性。最重要的是，成熟度指数经常有助于为乍一看似乎矛盾的结果找到适当的解释。结果表明，组织学分析及其对种群适应度的预测能力目前被低估，应成为评估潜在EDCs的标准组成部分。(c) 2012 Elsevier B.V.版权所有
In July 2011, the Fish Sexual Development Test (FSDT) has officially been adopted as OECD test guideline 234 for the detection of endocrine disrupting chemicals (EDCs). Sex ratio and vitellogenin (VTG) induction are the mandatory endocrine endpoints within this test, whereas gonad staging is only included as an option. In the present study, five FSDTs with zebrafish (Danio rerio) were conducted with EDCs with different modes of action (17 alpha-ethinylestradiol, dihydrotestosterone, 17 beta-trenbolone, prochloraz and 4-tert-pentylphenol). Results document that not only sex ratio and VTG production of the exposed fish were massively affected, but also gonad maturation. As a novel approach for the quantification of gonad maturation in zebrafish, the maturity index was developed to allow not only an improved assessment of dose-dependent EDC-related effects on gonad maturation, but also statistical analysis of histological data. VTG induction and maturity index showed an excellent correlation for all five EDCs tested. Most importantly, the maturity index often helped to find appropriate interpretations for results that seemed contradictory at first sight. Results show that histological analyses and their predictive power for population fitness are currently Underestimated and should become a standard component in the evaluation of potential EDCs. (c) 2012 Elsevier B.V. All rights reserved.