Development of 1,8-naphthalimide dyes for rapid imaging of subcellular compartments in plants
Development of 1,8-naphthalimide dyes for rapid imaging of subcellular compartments in plants
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DOI：
10.1039/d1cc05798g
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发表时间：
2021-12-07
影响因子：
4.9
通讯作者：
Hagihara, Shinya
中科院分区：
文献类型：
--
作者：
Kusano, Shuhei;Nakamura, Sakuya;Hagihara, Shinya
在这里，我们报告了1，8-萘酰亚胺染料在植物活体细胞成像中的安装。我们开发了一系列基于1，8-萘二甲酰亚胺的探针，通过改变它们的光谱特性来照亮不同的亚细胞隔间。简单地将探针渗透到叶片中，就能迅速观察到叶绿体或液泡的结构。我们进一步证明，这些探针适用于监测自噬途径中细胞器的行为。
Here, we report the installation of 1,8-naphthalimide dyes in live cell imaging of plants. We developed a series of 1,8-naphthalimide-based probes that illuminate different subcellular compartments by altering their spectral characteristics. Simple infiltration of the probes into leaves rapidly visualized the structure of chloroplasts or the vacuole. We further demonstrated that these probes are applicable to monitor the organelle behaviors in an autophagy pathway.