ORIGIN OF POLYSACCHARIDE DEPOLYMERASE ASSOCIATED WITH BACTERIOPHAGE INFECTION
ORIGIN OF POLYSACCHARIDE DEPOLYMERASE ASSOCIATED WITH BACTERIOPHAGE INFECTION
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DOI：
10.1128/jvi.3.3.290-296.1969
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发表时间：
1969-01-01
影响因子：
5.4
通讯作者：
ORR, TE
中科院分区：
文献类型：
--
作者：
BARTELL, PF;ORR, TE
通过构建噬菌体生长曲线的分析表明，铜绿假单胞菌菌株B和BI感染噬菌体2后合成多糖解聚酶。解聚酶的生物合成动力学与噬菌体导向病毒体的形成速率密切相关，而感染实验条件的改变则反映在酶的产生上。感染其他假单胞菌84和1197，不导致解聚酶的合成。在未感染的培养物中未检测到该酶，并且未获得在未感染或感染细胞的提取物中存在酶活性抑制剂或激活剂的证据。使用氯霉素或营养缺陷型突变体（BIarg−）的实验结果表明，蛋白质从头合成对酶的生产至关重要。已分离出噬菌体2的各种突变体（pdp1、pdp2），其改变多糖解聚酶的合成。这些实验结果有力地支持了噬菌体基因组在这种酶的合成中的作用。
Analyses, by construction of phage growth curves, indicated that the polysaccharide depolymerase was synthesized byPseudomonas aeruginosastrains B and BI after infection with phage 2. The kinetics of biosynthesis of the depolymerase were found to parallel closely the rate of formation of phage-directed virions, and alterations in the experimental conditions of infection were reflected by alterations in the production of enzyme. Infection with otherPseudomonasphages, 84 and 1197, did not result in the synthesis of depolymerase. The enzyme was not detectable in uninfected cultures, and no evidence was obtained for the existence of inhibitors or activators of enzyme activity in extracts of uninfected or infected cells. The results of experiments employing chloramphenicol or an auxotorphic mutant (BIarg−) suggested that protein synthesis de novo was essential for production of the enzyme. Various mutants of phage 2 (pdp1,pdp2), which alter the synthesis of the polysaccharide depolymerase, have been isolated. These experimental results strongly support the role of the phage genome in the synthesis of this enzyme.