Delta activity independent of its activity as a ligand of Notch.
Delta activity independent of its activity as a ligand of Notch.
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DOI：
10.1186/1471-213x-5-6
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发表时间：
2005-03-10
影响因子：
--
通讯作者：
Wesley C
中科院分区：
文献类型：
--
作者：
Mok LP;Qin T;Bardot B;LeComte M;Homayouni A;Ahimou F;Wesley C
Delta、Notch 和 Scabrous 在果蝇发育过程中经常一起发挥作用，产生不同的细胞类型并细化组织模式。 Delta被称为触发Notch受体活性的配体。 Scabrous 已知可结合 Notch 并促进 Notch 活性以响应 Delta。目前尚不清楚 Scabrous 是否能结合 Delta，或者 Delta 是否具有除作为 Notch 配体的活性之外的活性。在体内清楚地确定这种结合或活性是非常困难的，因为所有Notch、Delta和Scabrous活性都需要以相互依赖的方式同时或相继进行。使用果蝇培养细胞，我们发现全长 Delta 在缺乏 Scabrous 或 Notch 的情况下促进 Daughterless 蛋白、边缘 RNA 和穿山甲 RNA 的积累。 Scabrous 结合 Delta 并抑制这种活性，尽管它增加了 Delta 细胞内结构域的水平。我们还表明，在没有 Delta 的情况下，Scabrous 可以促进 Notch 受体活性。 Delta的活性与其作为Notch配体的活性无关。 Scabrous 抑制了 Delta 的活动。 Scabrous 还可以促进依赖于 Delta 配体活性的 Notch 活性。因此，Notch、Delta 和 Scabrous 可能在发育过程中以复杂的组合或相互排斥的相互作用发挥作用。这里报告的数据将对理解这些体内相互作用有重要帮助。
Delta, Notch, and Scabrous often function together to make different cell types and refine tissue patterns during Drosophila development. Delta is known as the ligand that triggers Notch receptor activity. Scabrous is known to bind Notch and promote Notch activity in response to Delta. It is not known if Scabrous binds Delta or Delta has activity other than its activity as a ligand of Notch. It is very difficult to clearly determine this binding or activity in vivo as all Notch, Delta, and Scabrous activities are required simultaneously or successively in an inter-dependent manner. Using Drosophila cultured cells we show that the full length Delta promotes accumulation of Daughterless protein, fringe RNA, and pangolin RNA in the absence of Scabrous or Notch. Scabrous binds Delta and suppresses this activity even though it increases the level of the Delta intracellular domain. We also show that Scabrous can promote Notch receptor activity, in the absence of Delta. Delta has activity that is independent of its activity as a ligand of Notch. Scabrous suppresses this Delta activity. Scabrous also promotes Notch activity that is dependent on Delta's ligand activity. Thus, Notch, Delta, and Scabrous might function in complex combinatorial or mutually exclusive interactions during development. The data reported here will be of significant help in understanding these interactions in vivo.