The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium.
The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium.
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发表时间：
1985-06
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通讯作者：
T. Doetschman;H. Eistetter;Margot Katz;Werner Schmidt;R. Kemler
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作者：
T. Doetschman;H. Eistetter;Margot Katz;Werner Schmidt;R. Kemler
研究了小鼠胚泡来源的胚胎干细胞系的体外发育潜力。悬浮培养3 ~ 8天，细胞形成内胚层、基膜、中胚层和外胚层的复杂胚状体。许多胚胎在形态上与6至8天的卵柱期胚胎相似。从8至10天的培养约一半的胚状体扩展成大的囊性结构，含有甲胎蛋白和转铁蛋白，因此是analagous的内脏卵黄囊的植入后胚胎。大约三分之一的囊状胚状体发育成心肌，当在人脐带血清存在下培养时，30%发育成血岛，从而以非常高的频率表现出高水平的有组织发育。此外，观察到的大多数胚胎干细胞系表现出类似的特征。胚胎干细胞系的体外发育潜力和细胞表达这种潜力的一致性被提出作为开辟胚胎发生研究新途径的方面。
The in vitro developmental potential of mouse blastocyst-derived embryonic stem cell lines has been investigated. From 3 to 8 days of suspension culture the cells form complex embryoid bodies with endoderm, basal lamina, mesoderm and ectoderm. Many are morphologically similar to embryos of the 6- to 8-day egg-cylinder stage. From 8 to 10 days of culture about half of the embryoid bodies expand into large cystic structures containing alphafoetoprotein and transferrin, thus being analagous to the visceral yolk sac of the postimplantation embryo. Approximately one third of the cystic embryoid bodies develop myocardium and when cultured in the presence of human cord serum, 30% develop blood islands, thereby exhibiting a high level of organized development at a very high frequency. Furthermore, most embryonic stem cell lines observed exhibit similar characteristics. The in vitro developmental potential of embryonic stem cell lines and the consistency with which the cells express this potential are presented as aspects which open up new approaches to the investigation of embryogenesis.