Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity
Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity
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DOI：
10.1039/b307024g
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发表时间：
2003-01-01
期刊：
影响因子：
4.2
通讯作者：
Wightman, RM
中科院分区：
文献类型：
--
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
比较了阳极电压为1.0V和1.4V的碳纤维微电极的伏安响应。快速扫描循环伏安法被用来表征对多巴胺和其他几种神经化学物质的反应。碳纤维吸附性能的提高导致体内灵敏度增加9倍。但是，随着阳极极限值的增大，传感器的时间响应变慢。电子转移动力学的增加也会导致多巴胺相对于其他神经化学物质的相对敏感性降低，以及它们的循环伏安图发生变化。使用Ro-04-1284和pargyline对尾壳核的刺激释放进行了体内药理学表征，并与多巴胺的预期结果一致。
The voltammetric responses of carbon-fiber microelectrodes with a 1.0 V and a 1.4 V anodic limit were compared in this study. Fast-scan cyclic voltammetry was used to characterize the response to dopamine and several other neurochemicals. An increase in the adsorption properties of the carbon fiber leads to an increase in sensitivity of 9 fold in vivo. However the temporal response of the sensor is slower with the more positive anodic limit. Increased electron transfer kinetics also causes a decrease in the relative sensitivity for dopamine vs. other neurochemicals, and a change in their cyclic voltammograms. Stimulated release in the caudate-putamen was pharmacologically characterized in vivo using Ro-04-1284 and pargyline, and was consistent with that expected for dopamine.